Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18032 | 3' | -54.7 | NC_004665.1 | + | 10098 | 0.66 | 0.572523 |
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Target: 5'- uACUGGgugGGUCGUGuCUGGCAcCCgaUCAa -3' miRNA: 3'- cUGACCa--UCGGCAC-GACUGUaGG--AGU- -5' |
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| 18032 | 3' | -54.7 | NC_004665.1 | + | 28652 | 0.67 | 0.527617 |
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Target: 5'- uGACgUGGaAGCUGUGCUGGCucgCCa-- -3' miRNA: 3'- -CUG-ACCaUCGGCACGACUGua-GGagu -5' |
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| 18032 | 3' | -54.7 | NC_004665.1 | + | 23931 | 0.69 | 0.42226 |
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Target: 5'- cACUGG-AGCCGuUGUUGACGUugaUCUCGa -3' miRNA: 3'- cUGACCaUCGGC-ACGACUGUA---GGAGU- -5' |
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| 18032 | 3' | -54.7 | NC_004665.1 | + | 2719 | 0.71 | 0.322264 |
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Target: 5'- aGCgUGGUGGUCGUGCUGugAaCCUg- -3' miRNA: 3'- cUG-ACCAUCGGCACGACugUaGGAgu -5' |
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| 18032 | 3' | -54.7 | NC_004665.1 | + | 8994 | 0.74 | 0.204386 |
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Target: 5'- cGCUGGUGGCUGUGCccgUGACcuguUCUUCGg -3' miRNA: 3'- cUGACCAUCGGCACG---ACUGu---AGGAGU- -5' |
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| 18032 | 3' | -54.7 | NC_004665.1 | + | 35259 | 1.08 | 0.000682 |
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Target: 5'- uGACUGGUAGCCGUGCUGACAUCCUCAu -3' miRNA: 3'- -CUGACCAUCGGCACGACUGUAGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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