Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18032 | 5' | -51.2 | NC_004665.1 | + | 29500 | 0.66 | 0.775388 |
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Target: 5'- cGUUGAG--UCCuCGAUAGCUUccGCCACg -3' miRNA: 3'- -CAACUCcaAGG-GUUGUUGAGa-CGGUG- -5' |
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| 18032 | 5' | -51.2 | NC_004665.1 | + | 28408 | 0.66 | 0.764531 |
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Target: 5'- aGUUGAccagCCCAGcCAGCUUUGCCGa -3' miRNA: 3'- -CAACUccaaGGGUU-GUUGAGACGGUg -5' |
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| 18032 | 5' | -51.2 | NC_004665.1 | + | 24696 | 0.67 | 0.731077 |
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Target: 5'- -cUGAGGacgCCCAACGccgaUCUGCC-Cg -3' miRNA: 3'- caACUCCaa-GGGUUGUug--AGACGGuG- -5' |
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| 18032 | 5' | -51.2 | NC_004665.1 | + | 19970 | 0.69 | 0.590686 |
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Target: 5'- cUUGGGGUcaUCCCAAagAACUCUcaugcaGCCAUa -3' miRNA: 3'- cAACUCCA--AGGGUUg-UUGAGA------CGGUG- -5' |
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| 18032 | 5' | -51.2 | NC_004665.1 | + | 21918 | 0.72 | 0.424062 |
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Target: 5'- -aUGAGGUgaaCCAGCGGCUUggUGCCGa -3' miRNA: 3'- caACUCCAag-GGUUGUUGAG--ACGGUg -5' |
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| 18032 | 5' | -51.2 | NC_004665.1 | + | 35296 | 1.1 | 0.001085 |
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Target: 5'- cGUUGAGGUUCCCAACAACUCUGCCACu -3' miRNA: 3'- -CAACUCCAAGGGUUGUUGAGACGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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