miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
18040 3' -44.7 NC_004667.1 + 12691 0.67 0.947602
Target:  5'- gCCGAGAAGUugGUG--CGCCGu--- -3'
miRNA:   3'- gGGCUUUUUAugCAUaaGCGGUgucg -5'
18040 3' -44.7 NC_004667.1 + 14360 0.68 0.91627
Target:  5'- uCCCGGA---UACGU--UCGCC-CAGa -3'
miRNA:   3'- -GGGCUUuuuAUGCAuaAGCGGuGUCg -5'
18040 3' -44.7 NC_004667.1 + 3181 0.69 0.879368
Target:  5'- aCCUguGGAAAAgaacgACGUAaugccagaaaaggccUUCGUCGCAGCc -3'
miRNA:   3'- -GGG--CUUUUUa----UGCAU---------------AAGCGGUGUCG- -5'
18040 3' -44.7 NC_004667.1 + 709 0.7 0.826982
Target:  5'- --aGGAAGugGUACGUGgaCGCCaACAGCu -3'
miRNA:   3'- gggCUUUU--UAUGCAUaaGCGG-UGUCG- -5'
18040 3' -44.7 NC_004667.1 + 4236 0.7 0.816338
Target:  5'- aCCCGAcgGAUcaccuUGUcuuucCGCCACAGCu -3'
miRNA:   3'- -GGGCUuuUUAu----GCAuaa--GCGGUGUCG- -5'
18040 3' -44.7 NC_004667.1 + 13417 1.18 0.0011
Target:  5'- gCCCGAAAAAUACGUAUUCGCCACAGCa -3'
miRNA:   3'- -GGGCUUUUUAUGCAUAAGCGGUGUCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.