Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18040 | 3' | -44.7 | NC_004667.1 | + | 12691 | 0.67 | 0.947602 |
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Target: 5'- gCCGAGAAGUugGUG--CGCCGu--- -3' miRNA: 3'- gGGCUUUUUAugCAUaaGCGGUgucg -5' |
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| 18040 | 3' | -44.7 | NC_004667.1 | + | 14360 | 0.68 | 0.91627 |
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Target: 5'- uCCCGGA---UACGU--UCGCC-CAGa -3' miRNA: 3'- -GGGCUUuuuAUGCAuaAGCGGuGUCg -5' |
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| 18040 | 3' | -44.7 | NC_004667.1 | + | 3181 | 0.69 | 0.879368 |
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Target: 5'- aCCUguGGAAAAgaacgACGUAaugccagaaaaggccUUCGUCGCAGCc -3' miRNA: 3'- -GGG--CUUUUUa----UGCAU---------------AAGCGGUGUCG- -5' |
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| 18040 | 3' | -44.7 | NC_004667.1 | + | 709 | 0.7 | 0.826982 |
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Target: 5'- --aGGAAGugGUACGUGgaCGCCaACAGCu -3' miRNA: 3'- gggCUUUU--UAUGCAUaaGCGG-UGUCG- -5' |
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| 18040 | 3' | -44.7 | NC_004667.1 | + | 4236 | 0.7 | 0.816338 |
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Target: 5'- aCCCGAcgGAUcaccuUGUcuuucCGCCACAGCu -3' miRNA: 3'- -GGGCUuuUUAu----GCAuaa--GCGGUGUCG- -5' |
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| 18040 | 3' | -44.7 | NC_004667.1 | + | 13417 | 1.18 | 0.0011 |
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Target: 5'- gCCCGAAAAAUACGUAUUCGCCACAGCa -3' miRNA: 3'- -GGGCUUUUUAUGCAUAAGCGGUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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