Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18047 | 5' | -56.3 | NC_004680.1 | + | 27005 | 0.66 | 0.717929 |
Target: 5'- aCCAGGGUu---CGCCGUUgGGUCCg- -3' miRNA: 3'- -GGUCCCGgcuuGUGGUAGgCUAGGag -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 45037 | 0.66 | 0.707398 |
Target: 5'- -aAGGGCCGAAgcaugaGCCG-CCGGUUCa- -3' miRNA: 3'- ggUCCCGGCUUg-----UGGUaGGCUAGGag -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 47576 | 0.66 | 0.707398 |
Target: 5'- gUCAGGGuuuCCGAggccaGC-CCAUCCGAggaCCUUc -3' miRNA: 3'- -GGUCCC---GGCU-----UGuGGUAGGCUa--GGAG- -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 17311 | 0.66 | 0.701045 |
Target: 5'- aCCAGGaucagccgcuCCGAuugcgccacaccgggGCACCGUCCGAUCa-- -3' miRNA: 3'- -GGUCCc---------GGCU---------------UGUGGUAGGCUAGgag -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 19253 | 0.66 | 0.686133 |
Target: 5'- aCAGGuuccGgCGGugACCAUCaCGAUCCg- -3' miRNA: 3'- gGUCC----CgGCUugUGGUAG-GCUAGGag -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 55337 | 0.66 | 0.686133 |
Target: 5'- gCCcGGGCUGcGCACCAUCaguGUCCc- -3' miRNA: 3'- -GGuCCCGGCuUGUGGUAGgc-UAGGag -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 30425 | 0.66 | 0.686133 |
Target: 5'- gCCAGcGCaguacGACACCAagcguucCCGAUCCUCa -3' miRNA: 3'- -GGUCcCGgc---UUGUGGUa------GGCUAGGAG- -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 26096 | 0.66 | 0.664669 |
Target: 5'- cCCGcGGCCccgccggcugGAACGCCA-CCG-UCCUCu -3' miRNA: 3'- -GGUcCCGG----------CUUGUGGUaGGCuAGGAG- -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 14183 | 0.67 | 0.632277 |
Target: 5'- uCC-GGGCCGAGuucacUACCGgugCCGGuuUCCUUg -3' miRNA: 3'- -GGuCCCGGCUU-----GUGGUa--GGCU--AGGAG- -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 33331 | 0.67 | 0.632277 |
Target: 5'- aUCGGGGCCucccGCACCGccgagCUGGUCCa- -3' miRNA: 3'- -GGUCCCGGcu--UGUGGUa----GGCUAGGag -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 29814 | 0.68 | 0.589125 |
Target: 5'- gCCAGGugcgcaucGCCGAagguguGCGCaacaaaAUCCuGAUCCUCg -3' miRNA: 3'- -GGUCC--------CGGCU------UGUGg-----UAGG-CUAGGAG- -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 41411 | 0.68 | 0.589125 |
Target: 5'- gCCGGGGCCGAugAUGCCGgcggCGAUCa-- -3' miRNA: 3'- -GGUCCCGGCU--UGUGGUag--GCUAGgag -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 50163 | 0.68 | 0.589125 |
Target: 5'- --cGGGUCGcggucGCACCGggCCGGUCCUa -3' miRNA: 3'- gguCCCGGCu----UGUGGUa-GGCUAGGAg -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 21421 | 0.69 | 0.536018 |
Target: 5'- aCGGGGCUGAGC-CCG-CgCGGUCC-Ca -3' miRNA: 3'- gGUCCCGGCUUGuGGUaG-GCUAGGaG- -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 33236 | 0.69 | 0.513164 |
Target: 5'- aCC-GGGCCGccauccacaaccACAUCAacCCGAUCCUCg -3' miRNA: 3'- -GGuCCCGGCu-----------UGUGGUa-GGCUAGGAG- -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 12712 | 0.69 | 0.504953 |
Target: 5'- aCCAGcGCCGggUGCCAUCgCG-UCCa- -3' miRNA: 3'- -GGUCcCGGCuuGUGGUAG-GCuAGGag -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 20746 | 0.69 | 0.501888 |
Target: 5'- gCCGauGGGCCGGgucaacaaucuccaGCACCAUgCCGAUCa-- -3' miRNA: 3'- -GGU--CCCGGCU--------------UGUGGUA-GGCUAGgag -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 28319 | 0.7 | 0.464802 |
Target: 5'- gUCGGGGCCGAucCACCAgggaacaCCGGUgCCg- -3' miRNA: 3'- -GGUCCCGGCUu-GUGGUa------GGCUA-GGag -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 3388 | 0.73 | 0.308097 |
Target: 5'- uCCA-GGCCGAucuuCGCUAUcgacaaCCGAUCCUCa -3' miRNA: 3'- -GGUcCCGGCUu---GUGGUA------GGCUAGGAG- -5' |
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18047 | 5' | -56.3 | NC_004680.1 | + | 5436 | 0.75 | 0.22806 |
Target: 5'- gCCAGGGCCGuGGCACCcgCaCGGguguaggCCUCc -3' miRNA: 3'- -GGUCCCGGC-UUGUGGuaG-GCUa------GGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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