Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18048 | 3' | -53.9 | NC_004680.1 | + | 31625 | 0.66 | 0.845851 |
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Target: 5'- cGCCGUCGgcgaacaccucuccAACCUGUUucccguccgGCACUGGcacACCGg -3' miRNA: 3'- -UGGCAGU--------------UUGGGCAG---------UGUGGCU---UGGC- -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 41742 | 0.66 | 0.839805 |
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Target: 5'- cACCGcCGAccccGCCCGcCGCaucuGCUGGACCu -3' miRNA: 3'- -UGGCaGUU----UGGGCaGUG----UGGCUUGGc -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 20590 | 0.66 | 0.838932 |
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Target: 5'- gGCCGUCAAAcuucuauCCCG--GCACCGAuuauCUGu -3' miRNA: 3'- -UGGCAGUUU-------GGGCagUGUGGCUu---GGC- -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 15863 | 0.66 | 0.821958 |
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Target: 5'- aGCCGUac-GCCuauuuCGUgGCGCCuGAGCCGg -3' miRNA: 3'- -UGGCAguuUGG-----GCAgUGUGG-CUUGGC- -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 42709 | 0.66 | 0.821958 |
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Target: 5'- uGCaGUCGAACCgCGUUGCGCCGcaaguuccCCGg -3' miRNA: 3'- -UGgCAGUUUGG-GCAGUGUGGCuu------GGC- -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 57340 | 0.66 | 0.812735 |
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Target: 5'- gGCCGgCGAACCUGaaacgCGuCACCGAACa- -3' miRNA: 3'- -UGGCaGUUUGGGCa----GU-GUGGCUUGgc -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 52060 | 0.75 | 0.331425 |
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Target: 5'- aACUGUgcgaAGACCCGUCGCA-CGGACCGc -3' miRNA: 3'- -UGGCAg---UUUGGGCAGUGUgGCUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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