Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18048 | 3' | -53.9 | NC_004680.1 | + | 19596 | 0.71 | 0.538784 |
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Target: 5'- cACCGgc-GGCgCCGUCAC-CCGGGCCa -3' miRNA: 3'- -UGGCaguUUG-GGCAGUGuGGCUUGGc -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 17192 | 0.72 | 0.507328 |
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Target: 5'- gACCGUguAACCgGaCACGCgGGGCCGc -3' miRNA: 3'- -UGGCAguUUGGgCaGUGUGgCUUGGC- -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 47857 | 0.73 | 0.456763 |
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Target: 5'- cGCCGgagucuCCCGuUCGCACCGAucccGCCGg -3' miRNA: 3'- -UGGCaguuu-GGGC-AGUGUGGCU----UGGC- -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 57018 | 0.73 | 0.427755 |
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Target: 5'- uGCCcuuGUCGAAUCCGUCGCGCCacuucuCCGg -3' miRNA: 3'- -UGG---CAGUUUGGGCAGUGUGGcuu---GGC- -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 40913 | 0.74 | 0.409032 |
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Target: 5'- uACCGUCGgcGACCCG-CACACCGugguagucgcACCa -3' miRNA: 3'- -UGGCAGU--UUGGGCaGUGUGGCu---------UGGc -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 52060 | 0.75 | 0.331425 |
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Target: 5'- aACUGUgcgaAGACCCGUCGCA-CGGACCGc -3' miRNA: 3'- -UGGCAg---UUUGGGCAGUGUgGCUUGGC- -5' |
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| 18048 | 3' | -53.9 | NC_004680.1 | + | 948 | 1.02 | 0.005913 |
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Target: 5'- -gCGUCAAACCCGUCACACCGAACCGg -3' miRNA: 3'- ugGCAGUUUGGGCAGUGUGGCUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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