Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18049 | 5' | -54.1 | NC_004680.1 | + | 5252 | 0.66 | 0.785786 |
Target: 5'- -cGACA-CCGGcguCGAUCUGCUCa--- -3' miRNA: 3'- caCUGUuGGCCu--GCUAGGCGAGgaau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 32371 | 0.66 | 0.755205 |
Target: 5'- uUGAaAGCCGGACacuGGUCCGCgCCa-- -3' miRNA: 3'- cACUgUUGGCCUG---CUAGGCGaGGaau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 47342 | 0.67 | 0.723474 |
Target: 5'- -gGACAGCCGGACGccCUuaUCCg-- -3' miRNA: 3'- caCUGUUGGCCUGCuaGGcgAGGaau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 18828 | 0.67 | 0.712696 |
Target: 5'- -cGACAACgGGAaguUGGaCCGCUCCg-- -3' miRNA: 3'- caCUGUUGgCCU---GCUaGGCGAGGaau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 5184 | 0.67 | 0.711614 |
Target: 5'- uUGGCGAaaacgguCUGGGCGGUCCaCUCCUc- -3' miRNA: 3'- cACUGUU-------GGCCUGCUAGGcGAGGAau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 51601 | 0.68 | 0.679924 |
Target: 5'- -cGGCGugCGccuuGGCGAUCCGCUCa--- -3' miRNA: 3'- caCUGUugGC----CUGCUAGGCGAGgaau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 47794 | 0.69 | 0.624522 |
Target: 5'- -cGGCAA-CGGACGAaacCCGCUCCc-- -3' miRNA: 3'- caCUGUUgGCCUGCUa--GGCGAGGaau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 49931 | 0.69 | 0.591277 |
Target: 5'- uGUGAUuuCCGcGACGGUUCGCUCg--- -3' miRNA: 3'- -CACUGuuGGC-CUGCUAGGCGAGgaau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 30873 | 0.69 | 0.580254 |
Target: 5'- -cGGucGCCGG-UGAUCCGCUCCa-- -3' miRNA: 3'- caCUguUGGCCuGCUAGGCGAGGaau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 51209 | 0.7 | 0.53668 |
Target: 5'- --cGCAGCCGGuCGAaaUCCGUUCCg-- -3' miRNA: 3'- cacUGUUGGCCuGCU--AGGCGAGGaau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 42574 | 0.7 | 0.515312 |
Target: 5'- -cGACAACgccACGAUCCGCUCCa-- -3' miRNA: 3'- caCUGUUGgccUGCUAGGCGAGGaau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 48390 | 0.72 | 0.430896 |
Target: 5'- gGUGACGAUguuuguaucgagcgCGGAUGAUCCGCgugUCCUg- -3' miRNA: 3'- -CACUGUUG--------------GCCUGCUAGGCG---AGGAau -5' |
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18049 | 5' | -54.1 | NC_004680.1 | + | 1119 | 1.06 | 0.00213 |
Target: 5'- gGUGACAACCGGACGAUCCGCUCCUUAc -3' miRNA: 3'- -CACUGUUGGCCUGCUAGGCGAGGAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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