Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18051 | 3' | -53.8 | NC_004680.1 | + | 12034 | 0.66 | 0.837214 |
Target: 5'- uGCCCGaUgGCuuugUUCGG-GACCAUGAu -3' miRNA: 3'- -CGGGC-AgCGuua-GAGUCaCUGGUGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 54527 | 0.66 | 0.828281 |
Target: 5'- uGCUCGUCGguGgcuUCAGguuCCACGAa -3' miRNA: 3'- -CGGGCAGCguUag-AGUCacuGGUGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 1715 | 0.67 | 0.790589 |
Target: 5'- -gCCGUCGC-AUCUCucc-ACCGCGAc -3' miRNA: 3'- cgGGCAGCGuUAGAGucacUGGUGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 19178 | 0.67 | 0.778737 |
Target: 5'- aGCCCGcCGUcGUCgauugugaaggcgUCGGUGACCcgcucggGCGAu -3' miRNA: 3'- -CGGGCaGCGuUAG-------------AGUCACUGG-------UGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 11535 | 0.67 | 0.760556 |
Target: 5'- cGCCCG-CGCAuccgacaugcgcAUCUCGGcGGCC-UGAa -3' miRNA: 3'- -CGGGCaGCGU------------UAGAGUCaCUGGuGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 20528 | 0.67 | 0.750268 |
Target: 5'- gGCCCaUCGUGAUCgCGGUGccguucgggauGCCugGAa -3' miRNA: 3'- -CGGGcAGCGUUAGaGUCAC-----------UGGugCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 23297 | 0.68 | 0.73986 |
Target: 5'- uGUCCGguucggggGCGAgcaUCUCGGUGacGCCGCGAa -3' miRNA: 3'- -CGGGCag------CGUU---AGAGUCAC--UGGUGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 49073 | 0.68 | 0.697259 |
Target: 5'- uGCCuCGUCGUAGUCaagcgCGGUGACUucuuCGu -3' miRNA: 3'- -CGG-GCAGCGUUAGa----GUCACUGGu---GCu -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 43715 | 0.69 | 0.664607 |
Target: 5'- -aCCGUUGCAGUUcCuG-GACCGCGAa -3' miRNA: 3'- cgGGCAGCGUUAGaGuCaCUGGUGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 4297 | 0.69 | 0.64267 |
Target: 5'- cGUCCGcCGCGGUCUgCGaUGuCCGCGAa -3' miRNA: 3'- -CGGGCaGCGUUAGA-GUcACuGGUGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 17950 | 0.7 | 0.620694 |
Target: 5'- aCCCGUCGCAuUCUCcAGcGGCgAUGGu -3' miRNA: 3'- cGGGCAGCGUuAGAG-UCaCUGgUGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 38026 | 0.7 | 0.598758 |
Target: 5'- cGCCCGcagaCGCucaacCUCAGUGACCaguucgGCGAc -3' miRNA: 3'- -CGGGCa---GCGuua--GAGUCACUGG------UGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 51668 | 0.72 | 0.502376 |
Target: 5'- gGUCCG-CGCAuacCUCGGUGAUgCACGAa -3' miRNA: 3'- -CGGGCaGCGUua-GAGUCACUG-GUGCU- -5' |
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18051 | 3' | -53.8 | NC_004680.1 | + | 1744 | 1.11 | 0.001315 |
Target: 5'- gGCCCGUCGCAAUCUCAGUGACCACGAc -3' miRNA: 3'- -CGGGCAGCGUUAGAGUCACUGGUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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