Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18052 | 5' | -56.3 | NC_004680.1 | + | 47660 | 0.7 | 0.438111 |
Target: 5'- -aCCACGGAUCGUAG-GCG-GCGCa- -3' miRNA: 3'- ggGGUGCCUAGCGUCaUGUaCGCGgu -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 47833 | 0.69 | 0.507635 |
Target: 5'- aUCCCGCcGGAUCGCAcGUGCA-GCaUCAa -3' miRNA: 3'- -GGGGUG-CCUAGCGU-CAUGUaCGcGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 59065 | 0.68 | 0.569627 |
Target: 5'- gCCCCAgCGGugugUGguGUguuggggGCAUGUGCCGg -3' miRNA: 3'- -GGGGU-GCCua--GCguCA-------UGUACGCGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 59311 | 0.66 | 0.700118 |
Target: 5'- gCCCGCGuucGAUCaGguGUucgaccCAUGCGCCu -3' miRNA: 3'- gGGGUGC---CUAG-CguCAu-----GUACGCGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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