Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18053 | 3' | -58.7 | NC_004680.1 | + | 11655 | 0.66 | 0.515811 |
Target: 5'- uUUCGGCCuauaaCCcCACucagGGCGGCCAc -3' miRNA: 3'- -AAGCCGGc----GGuGUGuaa-CCGUCGGUc -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 22550 | 0.66 | 0.505417 |
Target: 5'- aUCGGCaaugaCGCuuAUcGGCAGCCAGg -3' miRNA: 3'- aAGCCGgcg--GUGugUAaCCGUCGGUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 39396 | 0.66 | 0.464801 |
Target: 5'- gUUGGUCGUUgaccaucuGCACcagGGCGGCCAGu -3' miRNA: 3'- aAGCCGGCGG--------UGUGuaaCCGUCGGUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 42177 | 0.66 | 0.461822 |
Target: 5'- gUUGGaCCGCCACGCAgaucgccgaacagcUgGGCuGCCAc -3' miRNA: 3'- aAGCC-GGCGGUGUGU--------------AaCCGuCGGUc -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 34477 | 0.67 | 0.454912 |
Target: 5'- gUCGGCgaugcugacgauCGCC-CACGU--GCAGCCGGg -3' miRNA: 3'- aAGCCG------------GCGGuGUGUAacCGUCGGUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 50001 | 0.67 | 0.451968 |
Target: 5'- -gCGGaaaucacauucaccCCGCCGCGCAcccUGGCAGUgGGa -3' miRNA: 3'- aaGCC--------------GGCGGUGUGUa--ACCGUCGgUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 27909 | 0.67 | 0.425951 |
Target: 5'- -cCGGCCGCCGaGCu---GCGGCCAa -3' miRNA: 3'- aaGCCGGCGGUgUGuaacCGUCGGUc -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 27935 | 0.67 | 0.416543 |
Target: 5'- aUCGaCgGCCAUAUGUUGGUcuugGGCCGGg -3' miRNA: 3'- aAGCcGgCGGUGUGUAACCG----UCGGUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 43621 | 0.67 | 0.407264 |
Target: 5'- -cCGGCCGCC-CugGUcgaacgUGGCGGCg-- -3' miRNA: 3'- aaGCCGGCGGuGugUA------ACCGUCGguc -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 42298 | 0.68 | 0.389099 |
Target: 5'- --gGGCaCGCUGCACGgugcgguggUGGCAGCcCAGc -3' miRNA: 3'- aagCCG-GCGGUGUGUa--------ACCGUCG-GUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 53889 | 0.68 | 0.389099 |
Target: 5'- cUCGG-UGCCGCACAUcUGGCcGCCc- -3' miRNA: 3'- aAGCCgGCGGUGUGUA-ACCGuCGGuc -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 48681 | 0.68 | 0.379337 |
Target: 5'- gUCGGCCGCaaguaccuauaccUGCACggUGaCAGCCGGa -3' miRNA: 3'- aAGCCGGCG-------------GUGUGuaACcGUCGGUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 43536 | 0.68 | 0.361162 |
Target: 5'- ---cGCCGCCACGuucgaccagGGCGGCCGGg -3' miRNA: 3'- aagcCGGCGGUGUguaa-----CCGUCGGUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 12529 | 0.68 | 0.3544 |
Target: 5'- aUCgGGCCGCCGcCACcaccgGUggugGGCAGCgGGg -3' miRNA: 3'- aAG-CCGGCGGU-GUG-----UAa---CCGUCGgUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 27510 | 0.69 | 0.329844 |
Target: 5'- aUCGacGUCGCCGCAUAUcUGGCGGUgCAGg -3' miRNA: 3'- aAGC--CGGCGGUGUGUA-ACCGUCG-GUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 1965 | 0.69 | 0.321942 |
Target: 5'- -cUGGCUGCCAaugugUGGCGGCCGa -3' miRNA: 3'- aaGCCGGCGGUguguaACCGUCGGUc -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 50192 | 0.7 | 0.306566 |
Target: 5'- -cCGGCUGCuCGCcgaAUUGGUAGCCGa -3' miRNA: 3'- aaGCCGGCG-GUGug-UAACCGUCGGUc -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 27829 | 0.7 | 0.291758 |
Target: 5'- gUUGGCCGCa--GCuc-GGCGGCCGGa -3' miRNA: 3'- aAGCCGGCGgugUGuaaCCGUCGGUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 59153 | 0.72 | 0.208957 |
Target: 5'- cUUGGCgGCCGCAuUGUUGGCAGguCCAGu -3' miRNA: 3'- aAGCCGgCGGUGU-GUAACCGUC--GGUC- -5' |
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18053 | 3' | -58.7 | NC_004680.1 | + | 40261 | 0.72 | 0.208957 |
Target: 5'- gUUCcuGCCGCCuGCGCAUUGGgAGUCGGa -3' miRNA: 3'- -AAGc-CGGCGG-UGUGUAACCgUCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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