Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18054 | 5' | -50.4 | NC_004680.1 | + | 10054 | 0.69 | 0.804524 |
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Target: 5'- aCGAugUUGUUGaCCAGACGGCauUCg -3' miRNA: 3'- aGUUugAACAGCcGGUUUGCCGguAG- -5' |
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| 18054 | 5' | -50.4 | NC_004680.1 | + | 57495 | 0.7 | 0.763502 |
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Target: 5'- gUUGAACUgcUgGGuuCCAGGCGGCCGUCg -3' miRNA: 3'- -AGUUUGAacAgCC--GGUUUGCCGGUAG- -5' |
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| 18054 | 5' | -50.4 | NC_004680.1 | + | 22037 | 0.72 | 0.675317 |
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Target: 5'- cCAcGCgacUGUgGGCCAcGGCGGCCAUg -3' miRNA: 3'- aGUuUGa--ACAgCCGGU-UUGCCGGUAg -5' |
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| 18054 | 5' | -50.4 | NC_004680.1 | + | 548 | 0.72 | 0.662829 |
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Target: 5'- -gAAGCUUGUgaccugaCGGCCuuAUGGCUGUCu -3' miRNA: 3'- agUUUGAACA-------GCCGGuuUGCCGGUAG- -5' |
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| 18054 | 5' | -50.4 | NC_004680.1 | + | 33506 | 0.72 | 0.641179 |
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Target: 5'- cUCGggUUUGUCGaCCAAgGCGGCCAc- -3' miRNA: 3'- -AGUuuGAACAGCcGGUU-UGCCGGUag -5' |
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| 18054 | 5' | -50.4 | NC_004680.1 | + | 27181 | 0.72 | 0.629766 |
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Target: 5'- cCAGGCUucgucccauuUGUCGGCguAGCGGUCAg- -3' miRNA: 3'- aGUUUGA----------ACAGCCGguUUGCCGGUag -5' |
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| 18054 | 5' | -50.4 | NC_004680.1 | + | 20759 | 0.8 | 0.270407 |
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Target: 5'- aUCu-GCUUGaUCGGCCGAugGGCCggGUCa -3' miRNA: 3'- -AGuuUGAAC-AGCCGGUUugCCGG--UAG- -5' |
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| 18054 | 5' | -50.4 | NC_004680.1 | + | 2163 | 1.1 | 0.002649 |
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Target: 5'- aUCAAACUUGUCGGCCAAACGGCCAUCc -3' miRNA: 3'- -AGUUUGAACAGCCGGUUUGCCGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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