Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18055 | 5' | -52.6 | NC_004680.1 | + | 42642 | 0.66 | 0.823514 |
Target: 5'- cCUGGCCCAgGC--GGCCCGcCUGCUc- -3' miRNA: 3'- -GGUCGGGUaUGaaCUGGGU-GACGAau -5' |
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18055 | 5' | -52.6 | NC_004680.1 | + | 13929 | 0.67 | 0.784277 |
Target: 5'- gCGGCCCAUGCgc-GCCCACagGUc-- -3' miRNA: 3'- gGUCGGGUAUGaacUGGGUGa-CGaau -5' |
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18055 | 5' | -52.6 | NC_004680.1 | + | 15712 | 0.67 | 0.76361 |
Target: 5'- aCUGGCCCgacgaaaacgGUGCUUGAUUCGgUGCUg- -3' miRNA: 3'- -GGUCGGG----------UAUGAACUGGGUgACGAau -5' |
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18055 | 5' | -52.6 | NC_004680.1 | + | 21333 | 0.69 | 0.698585 |
Target: 5'- aCCAGCCCcgGCc-GACgaCACUGCa-- -3' miRNA: 3'- -GGUCGGGuaUGaaCUGg-GUGACGaau -5' |
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18055 | 5' | -52.6 | NC_004680.1 | + | 34232 | 0.69 | 0.676233 |
Target: 5'- cCCGGUCCGcaUGCgcauaGAUCCGCUGCg-- -3' miRNA: 3'- -GGUCGGGU--AUGaa---CUGGGUGACGaau -5' |
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18055 | 5' | -52.6 | NC_004680.1 | + | 11151 | 0.69 | 0.642403 |
Target: 5'- gCAGCCC-UGCgggUGACCCGauuCUGCc-- -3' miRNA: 3'- gGUCGGGuAUGa--ACUGGGU---GACGaau -5' |
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18055 | 5' | -52.6 | NC_004680.1 | + | 35099 | 0.7 | 0.585958 |
Target: 5'- gCCGGCCCGaGgUUGGCUCgGCUGCg-- -3' miRNA: 3'- -GGUCGGGUaUgAACUGGG-UGACGaau -5' |
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18055 | 5' | -52.6 | NC_004680.1 | + | 2629 | 1.08 | 0.001879 |
Target: 5'- uCCAGCCCAUACUUGACCCACUGCUUAc -3' miRNA: 3'- -GGUCGGGUAUGAACUGGGUGACGAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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