Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18056 | 3' | -49.9 | NC_004680.1 | + | 27871 | 0.69 | 0.827215 |
Target: 5'- --gCGCGAucggCgCGaaGCUCGGCAUCGAa -3' miRNA: 3'- uuaGCGUUuua-G-GC--UGAGCCGUAGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 12385 | 0.69 | 0.845636 |
Target: 5'- --cUGCcGGGUCCGGCUCcGGUGUCGu -3' miRNA: 3'- uuaGCGuUUUAGGCUGAG-CCGUAGCu -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 33204 | 0.68 | 0.863089 |
Target: 5'- --aCGUAAAGUCCGcCcCGGCGUCc- -3' miRNA: 3'- uuaGCGUUUUAGGCuGaGCCGUAGcu -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 1468 | 0.66 | 0.933746 |
Target: 5'- gGAUCGCGAAGgacuaCGACcguacCGGUAUUGAu -3' miRNA: 3'- -UUAGCGUUUUag---GCUGa----GCCGUAGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 44219 | 0.66 | 0.939192 |
Target: 5'- cAUCGCAcuGUCCGgGCcuagCGGCAgCGGg -3' miRNA: 3'- uUAGCGUuuUAGGC-UGa---GCCGUaGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 22064 | 0.66 | 0.944343 |
Target: 5'- uAUCGCGAugcguGGUCCGg--CGGCGuUCGAg -3' miRNA: 3'- uUAGCGUU-----UUAGGCugaGCCGU-AGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 36544 | 0.66 | 0.947773 |
Target: 5'- cGUCGCugucGAGUCCGagaagaagauguugGCUgCGGCgAUCGAa -3' miRNA: 3'- uUAGCGu---UUUAGGC--------------UGA-GCCG-UAGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 5242 | 0.79 | 0.325815 |
Target: 5'- --aCGCGAuuUCCGACacCGGCGUCGAu -3' miRNA: 3'- uuaGCGUUuuAGGCUGa-GCCGUAGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 2799 | 1.08 | 0.004595 |
Target: 5'- cAAUCGCAAAAUCCGACUCGGCAUCGAg -3' miRNA: 3'- -UUAGCGUUUUAGGCUGAGCCGUAGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 3301 | 0.74 | 0.587452 |
Target: 5'- -uUCGCGcuGUCCGGCaUCaGCAUCGGc -3' miRNA: 3'- uuAGCGUuuUAGGCUG-AGcCGUAGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 43417 | 0.7 | 0.7878 |
Target: 5'- aGGUCuCAAAGUCUucacagcaGACUCGGCAUaCGAc -3' miRNA: 3'- -UUAGcGUUUUAGG--------CUGAGCCGUA-GCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 14448 | 0.7 | 0.797947 |
Target: 5'- --aCGCAGgaacacAAUCUGAUUCGGCAgcagCGGa -3' miRNA: 3'- uuaGCGUU------UUAGGCUGAGCCGUa---GCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 25727 | 0.7 | 0.807906 |
Target: 5'- uGUCGCAGuGUuuGGgUgCGGCGUCGGc -3' miRNA: 3'- uUAGCGUUuUAggCUgA-GCCGUAGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 28336 | 0.68 | 0.887296 |
Target: 5'- --gCGCGGg--CgGugUCGGCGUCGGg -3' miRNA: 3'- uuaGCGUUuuaGgCugAGCCGUAGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 13660 | 0.67 | 0.902037 |
Target: 5'- uGUCGUAGuugaaCGugUCGGuCAUCGAc -3' miRNA: 3'- uUAGCGUUuuag-GCugAGCC-GUAGCU- -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 13137 | 0.67 | 0.928 |
Target: 5'- aGGUCGUAccAUCCGug-CGGCGUCa- -3' miRNA: 3'- -UUAGCGUuuUAGGCugaGCCGUAGcu -5' |
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18056 | 3' | -49.9 | NC_004680.1 | + | 43251 | 0.66 | 0.948727 |
Target: 5'- -cUUGCGGGAuucuuuuUCUGACU-GGCGUCGGc -3' miRNA: 3'- uuAGCGUUUU-------AGGCUGAgCCGUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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