Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18058 | 3' | -51.1 | NC_004680.1 | + | 34465 | 0.66 | 0.928777 |
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Target: 5'- gCCgccgCGGUAGUCg--GCgaugCUGACGAUc -3' miRNA: 3'- -GGaa--GCCGUUAGauaCGa---GGCUGCUG- -5' |
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| 18058 | 3' | -51.1 | NC_004680.1 | + | 42529 | 0.67 | 0.886961 |
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Target: 5'- aCCacCGGCGacaucgccaaagaaGUCggAUGCUCCGACcGCa -3' miRNA: 3'- -GGaaGCCGU--------------UAGa-UACGAGGCUGcUG- -5' |
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| 18058 | 3' | -51.1 | NC_004680.1 | + | 29049 | 0.67 | 0.881633 |
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Target: 5'- aCCgaUUGGguGUCaGUGCugucagugUCCGGCGGCg -3' miRNA: 3'- -GGa-AGCCguUAGaUACG--------AGGCUGCUG- -5' |
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| 18058 | 3' | -51.1 | NC_004680.1 | + | 3280 | 0.67 | 0.873796 |
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Target: 5'- --aUCGGCAgcggaGUCUugGUcgCCGACGACa -3' miRNA: 3'- ggaAGCCGU-----UAGAuaCGa-GGCUGCUG- -5' |
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| 18058 | 3' | -51.1 | NC_004680.1 | + | 14843 | 0.68 | 0.84788 |
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Target: 5'- gCCgcaUGGCAAUCg--GCccuccagUCCGGCGACc -3' miRNA: 3'- -GGaa-GCCGUUAGauaCG-------AGGCUGCUG- -5' |
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| 18058 | 3' | -51.1 | NC_004680.1 | + | 4543 | 0.69 | 0.821588 |
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Target: 5'- uCCguuaCGGCg--CUGUGUUCCGACGu- -3' miRNA: 3'- -GGaa--GCCGuuaGAUACGAGGCUGCug -5' |
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| 18058 | 3' | -51.1 | NC_004680.1 | + | 25392 | 0.69 | 0.812105 |
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Target: 5'- gCCggUUGGCGAUC-AUGCgUUCGAUGAUu -3' miRNA: 3'- -GGa-AGCCGUUAGaUACG-AGGCUGCUG- -5' |
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| 18058 | 3' | -51.1 | NC_004680.1 | + | 26037 | 0.72 | 0.641636 |
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Target: 5'- gCCUcagcgCGGCGGUCUAcGCgcuaggggcgaUCCGAUGACc -3' miRNA: 3'- -GGAa----GCCGUUAGAUaCG-----------AGGCUGCUG- -5' |
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| 18058 | 3' | -51.1 | NC_004680.1 | + | 3097 | 1.13 | 0.0018 |
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Target: 5'- aCCUUCGGCAAUCUAUGCUCCGACGACu -3' miRNA: 3'- -GGAAGCCGUUAGAUACGAGGCUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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