Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18059 | 3' | -58.7 | NC_004680.1 | + | 36258 | 0.66 | 0.554188 |
Target: 5'- -gCGCCaagacGGCUGCCgcgAGCgAGGGAUu -3' miRNA: 3'- gaGCGG-----CCGGCGGaa-UCGgUCUCUAc -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 51843 | 0.66 | 0.543663 |
Target: 5'- -gUGCUGcGCUGCCgcacacGCCAGAGGUc -3' miRNA: 3'- gaGCGGC-CGGCGGaau---CGGUCUCUAc -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 42079 | 0.66 | 0.533205 |
Target: 5'- gCUCGaaugaGuGCCGCCg-GGCgCAGAGGUGg -3' miRNA: 3'- -GAGCgg---C-CGGCGGaaUCG-GUCUCUAC- -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 57408 | 0.66 | 0.533205 |
Target: 5'- uUCGCCGGCCGgcguggauCCUUaccgcGGUCAcggaacGGGAUGa -3' miRNA: 3'- gAGCGGCCGGC--------GGAA-----UCGGU------CUCUAC- -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 52198 | 0.67 | 0.49216 |
Target: 5'- -gCGCCGGCaaGCCgacgAGCCAGgAGGc- -3' miRNA: 3'- gaGCGGCCGg-CGGaa--UCGGUC-UCUac -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 53896 | 0.67 | 0.491151 |
Target: 5'- uCUCGCCGGCguugucgCGCCUguaGGUguG-GGUGg -3' miRNA: 3'- -GAGCGGCCG-------GCGGAa--UCGguCuCUAC- -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 34462 | 0.67 | 0.462355 |
Target: 5'- uUCGCC-GCCGCggUAGUCGGcGAUGc -3' miRNA: 3'- gAGCGGcCGGCGgaAUCGGUCuCUAC- -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 8939 | 0.67 | 0.452632 |
Target: 5'- --gGUCGuGCUGCUgguGCCAGGGAUGu -3' miRNA: 3'- gagCGGC-CGGCGGaauCGGUCUCUAC- -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 47841 | 0.67 | 0.442066 |
Target: 5'- -gCGCCGGCUGgCUgcggcgguUGGCCGggucgcuGAGAUGa -3' miRNA: 3'- gaGCGGCCGGCgGA--------AUCGGU-------CUCUAC- -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 22087 | 0.68 | 0.433527 |
Target: 5'- gUgGCUGGCCGCCcccauccCCAGAGAa- -3' miRNA: 3'- gAgCGGCCGGCGGaauc---GGUCUCUac -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 44875 | 0.71 | 0.283506 |
Target: 5'- cCUCccCCGGCaucggcacaacaGCCUUGGCCAGAGuGUGg -3' miRNA: 3'- -GAGc-GGCCGg-----------CGGAAUCGGUCUC-UAC- -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 21210 | 0.72 | 0.239018 |
Target: 5'- aCUgGCCGguGCCGCCggAGCCGGuGGAUa -3' miRNA: 3'- -GAgCGGC--CGGCGGaaUCGGUC-UCUAc -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 42719 | 0.73 | 0.194311 |
Target: 5'- --gGCgGGCCGCCUgGGCCAGgacauguucagGGAUGg -3' miRNA: 3'- gagCGgCCGGCGGAaUCGGUC-----------UCUAC- -5' |
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18059 | 3' | -58.7 | NC_004680.1 | + | 3525 | 1.08 | 0.000597 |
Target: 5'- aCUCGCCGGCCGCCUUAGCCAGAGAUGc -3' miRNA: 3'- -GAGCGGCCGGCGGAAUCGGUCUCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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