Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18060 | 5' | -59.6 | NC_004680.1 | + | 3800 | 1.08 | 0.00043 |
Target: 5'- uUGGGCCGCUUGGACAACCGGUGCCACc -3' miRNA: 3'- -ACCCGGCGAACCUGUUGGCCACGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 28316 | 0.78 | 0.081505 |
Target: 5'- gGGGCCGauccaccagGGAacACCGGUGCCGCa -3' miRNA: 3'- aCCCGGCgaa------CCUguUGGCCACGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 11189 | 0.71 | 0.25165 |
Target: 5'- -cGGCCGCggcguucugcUUGGGC-GCCGGgGCCGCc -3' miRNA: 3'- acCCGGCG----------AACCUGuUGGCCaCGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 14181 | 0.69 | 0.329486 |
Target: 5'- cGGGCCGaguucACuACCGGUGCCGg -3' miRNA: 3'- aCCCGGCgaaccUGuUGGCCACGGUg -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 12527 | 0.68 | 0.354408 |
Target: 5'- cGGGCCGCcgccaccaccgguggUGGGCAGCgGG-GCUg- -3' miRNA: 3'- aCCCGGCGa--------------ACCUGUUGgCCaCGGug -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 14936 | 0.68 | 0.370328 |
Target: 5'- gGGGUCGCcggacUGGAgGGCCGauUGCCAUg -3' miRNA: 3'- aCCCGGCGa----ACCUgUUGGCc-ACGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 53704 | 0.67 | 0.414496 |
Target: 5'- cGGGCgaUGgUUGGAgguacgccuuugUAGCCGGguUGCCACa -3' miRNA: 3'- aCCCG--GCgAACCU------------GUUGGCC--ACGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 28386 | 0.66 | 0.471546 |
Target: 5'- aUGGuGCUGC-UGGGUAGCCgaugGGuUGCCACa -3' miRNA: 3'- -ACC-CGGCGaACCUGUUGG----CC-ACGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 28269 | 0.66 | 0.481441 |
Target: 5'- cGGGCCGggUGuccACGACCG--GCCGCg -3' miRNA: 3'- aCCCGGCgaACc--UGUUGGCcaCGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 25780 | 0.66 | 0.498489 |
Target: 5'- gGGGCCuguggcgcaggcguGUUUGuaGAUgaGGCCGGUGCCGg -3' miRNA: 3'- aCCCGG--------------CGAAC--CUG--UUGGCCACGGUg -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 41053 | 0.66 | 0.501525 |
Target: 5'- uUGGGgUGCggcUGGAUgAGCUuGUGCCGCu -3' miRNA: 3'- -ACCCgGCGa--ACCUG-UUGGcCACGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 23081 | 0.74 | 0.149254 |
Target: 5'- cUGGcGCCGC-UGGcACGACCGGggcgaaugaaGCCACg -3' miRNA: 3'- -ACC-CGGCGaACC-UGUUGGCCa---------CGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 37847 | 0.66 | 0.501525 |
Target: 5'- gGGGCCGCacuccgugugUGGAgAucgaaguggucACCGGcaugacUGCCGCc -3' miRNA: 3'- aCCCGGCGa---------ACCUgU-----------UGGCC------ACGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 31407 | 0.66 | 0.501525 |
Target: 5'- cGuGGCCGCgUGGAgGGagcaCGGUcacuaguucgGCCACg -3' miRNA: 3'- aC-CCGGCGaACCUgUUg---GCCA----------CGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 2833 | 0.66 | 0.471546 |
Target: 5'- gUGGGuuCCGgUUGGugAugcuguagugACCGGUucGCCGCc -3' miRNA: 3'- -ACCC--GGCgAACCugU----------UGGCCA--CGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 24868 | 0.66 | 0.452073 |
Target: 5'- cGGG-CGCccccggagUGGAUAACacuaguggCGGUGCCGCc -3' miRNA: 3'- aCCCgGCGa-------ACCUGUUG--------GCCACGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 51809 | 0.67 | 0.414496 |
Target: 5'- cGGGCCGCggUGuucGCAAuagccaaugcucUCGGUGCUGCg -3' miRNA: 3'- aCCCGGCGa-ACc--UGUU------------GGCCACGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 14130 | 0.67 | 0.414496 |
Target: 5'- gGGGCaucggGUUUGGuauGCAgGCCGGUGgCGCg -3' miRNA: 3'- aCCCGg----CGAACC---UGU-UGGCCACgGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 5904 | 0.67 | 0.396441 |
Target: 5'- cUGcGG-CGCUUGGAUuGCCGaGcGCCGCa -3' miRNA: 3'- -AC-CCgGCGAACCUGuUGGC-CaCGGUG- -5' |
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18060 | 5' | -59.6 | NC_004680.1 | + | 36171 | 0.68 | 0.378901 |
Target: 5'- aGGGCgGCgaGGACcGCUGGUcuGCuCGCu -3' miRNA: 3'- aCCCGgCGaaCCUGuUGGCCA--CG-GUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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