Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18061 | 3' | -61.7 | NC_004680.1 | + | 11389 | 0.66 | 0.391775 |
Target: 5'- ---aGAGGACCGGCUCAagGCC-CGUa- -3' miRNA: 3'- uacgCUCCUGGCCGGGU--UGGaGCGgu -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 3290 | 0.66 | 0.386564 |
Target: 5'- aGUGUGAGGAUCGGUugucgauagcgaagaUCGGCCUggagGCCAu -3' miRNA: 3'- -UACGCUCCUGGCCG---------------GGUUGGAg---CGGU- -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 41331 | 0.66 | 0.383116 |
Target: 5'- -cGCcGGcaucAUCGGcCCCGGCCUCGCCGc -3' miRNA: 3'- uaCGcUCc---UGGCC-GGGUUGGAGCGGU- -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 27364 | 0.66 | 0.383116 |
Target: 5'- -gGCGAGGuucUCGGCggCAGCgUCGCCGc -3' miRNA: 3'- uaCGCUCCu--GGCCGg-GUUGgAGCGGU- -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 58467 | 0.67 | 0.349775 |
Target: 5'- -cGCcuacACCuGGCCCAGCCUCGUCGa -3' miRNA: 3'- uaCGcuccUGG-CCGGGUUGGAGCGGU- -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 21334 | 0.67 | 0.341769 |
Target: 5'- -gGCGuGGGACCgcgcgGGCUCAGCCcCGUCGg -3' miRNA: 3'- uaCGC-UCCUGG-----CCGGGUUGGaGCGGU- -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 55804 | 0.67 | 0.341769 |
Target: 5'- -cGCGAuGGCCGGuaaaacCCCAACCgccacaCGCCGa -3' miRNA: 3'- uaCGCUcCUGGCC------GGGUUGGa-----GCGGU- -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 54050 | 0.67 | 0.326157 |
Target: 5'- cUGCGAGGA-CGGCCgCGAguaCUaCGCCGu -3' miRNA: 3'- uACGCUCCUgGCCGG-GUUg--GA-GCGGU- -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 50168 | 0.68 | 0.303016 |
Target: 5'- -cGCGGucgcaccGGGCCGGUCCuACCggcugcUCGCCGa -3' miRNA: 3'- uaCGCU-------CCUGGCCGGGuUGG------AGCGGU- -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 16850 | 0.68 | 0.269054 |
Target: 5'- -gGCGuGuACCGGUUgAACCUCGCCc -3' miRNA: 3'- uaCGCuCcUGGCCGGgUUGGAGCGGu -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 42018 | 0.69 | 0.249816 |
Target: 5'- cUGCGGGuGACCGGUUCGaacACCUCaCCGc -3' miRNA: 3'- uACGCUC-CUGGCCGGGU---UGGAGcGGU- -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 50263 | 0.69 | 0.237631 |
Target: 5'- -gGCGAgcagccgguaGGACCGGCCCGgugcgaccgcgACCcgagaccccuUCGCCAc -3' miRNA: 3'- uaCGCU----------CCUGGCCGGGU-----------UGG----------AGCGGU- -5' |
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18061 | 3' | -61.7 | NC_004680.1 | + | 4363 | 1.06 | 0.000412 |
Target: 5'- cAUGCGAGGACCGGCCCAACCUCGCCAu -3' miRNA: 3'- -UACGCUCCUGGCCGGGUUGGAGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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