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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18061 | 5' | -49.5 | NC_004680.1 | + | 39987 | 0.69 | 0.853398 |
Target: 5'- cGUG-CGAUCGACUUCCGuGUGaacUCGAu -3' miRNA: 3'- uCACuGCUAGUUGAAGGC-CAUc--AGUU- -5' |
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18061 | 5' | -49.5 | NC_004680.1 | + | 22651 | 0.72 | 0.687762 |
Target: 5'- aGGUGACGGUCAacaagcccaccgGCUUCgGGUGGg--- -3' miRNA: 3'- -UCACUGCUAGU------------UGAAGgCCAUCaguu -5' |
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18061 | 5' | -49.5 | NC_004680.1 | + | 4329 | 1.07 | 0.005692 |
Target: 5'- gAGUGACGAUCAACUUCCGGUAGUCAAc -3' miRNA: 3'- -UCACUGCUAGUUGAAGGCCAUCAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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