Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18062 | 3' | -53.1 | NC_004680.1 | + | 24041 | 0.66 | 0.848172 |
Target: 5'- uGC-CGGUCGAUGguucggugaCGUCGGCGAaGAu -3' miRNA: 3'- -UGuGCCAGCUACa--------GUAGCUGCUcCUu -5' |
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18062 | 3' | -53.1 | NC_004680.1 | + | 21238 | 0.66 | 0.848172 |
Target: 5'- uAUugGGuUCGAugcagUGUCGUCGGCcGGGGc -3' miRNA: 3'- -UGugCC-AGCU-----ACAGUAGCUGcUCCUu -5' |
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18062 | 3' | -53.1 | NC_004680.1 | + | 59200 | 0.67 | 0.782464 |
Target: 5'- uCGgGGUUGuAUGUgGUCGGCGuGGAGu -3' miRNA: 3'- uGUgCCAGC-UACAgUAGCUGCuCCUU- -5' |
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18062 | 3' | -53.1 | NC_004680.1 | + | 27466 | 0.69 | 0.676103 |
Target: 5'- aGCACGGUCGGUGUCGacgcaaUCGACc----- -3' miRNA: 3'- -UGUGCCAGCUACAGU------AGCUGcuccuu -5' |
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18062 | 3' | -53.1 | NC_004680.1 | + | 28337 | 0.7 | 0.642836 |
Target: 5'- gGCGCGGgCGGUGUCggCGuCGGGGc- -3' miRNA: 3'- -UGUGCCaGCUACAGuaGCuGCUCCuu -5' |
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18062 | 3' | -53.1 | NC_004680.1 | + | 15102 | 0.71 | 0.543538 |
Target: 5'- -gAUGGUCGGUGUCAUCGuCGAaaccgcGGGu -3' miRNA: 3'- ugUGCCAGCUACAGUAGCuGCU------CCUu -5' |
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18062 | 3' | -53.1 | NC_004680.1 | + | 37023 | 0.75 | 0.365584 |
Target: 5'- gGCGCGuUCGAgugCAUCGAUGAGGAAg -3' miRNA: 3'- -UGUGCcAGCUacaGUAGCUGCUCCUU- -5' |
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18062 | 3' | -53.1 | NC_004680.1 | + | 5383 | 1.06 | 0.002912 |
Target: 5'- aACACGGUCGAUGUCAUCGACGAGGAAg -3' miRNA: 3'- -UGUGCCAGCUACAGUAGCUGCUCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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