Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18062 | 5' | -48.8 | NC_004680.1 | + | 54971 | 0.66 | 0.964897 |
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Target: 5'- ---gCCggcacGAUCGGUG-GCGGGUCGGa -3' miRNA: 3'- aaaaGGa----CUAGCUACuCGUCUAGCUg -5' |
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| 18062 | 5' | -48.8 | NC_004680.1 | + | 44896 | 0.66 | 0.960637 |
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Target: 5'- ---cCCUGAUCuGGUGGGCgaacuccucccccGGcAUCGGCa -3' miRNA: 3'- aaaaGGACUAG-CUACUCG-------------UC-UAGCUG- -5' |
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| 18062 | 5' | -48.8 | NC_004680.1 | + | 5685 | 0.66 | 0.956895 |
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Target: 5'- ----aCUGA-CGAUGAGCAGuUCGuCg -3' miRNA: 3'- aaaagGACUaGCUACUCGUCuAGCuG- -5' |
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| 18062 | 5' | -48.8 | NC_004680.1 | + | 48427 | 0.66 | 0.956895 |
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Target: 5'- --gUCCUGGaggCGGUGucguGCAGGUCGu- -3' miRNA: 3'- aaaAGGACUa--GCUACu---CGUCUAGCug -5' |
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| 18062 | 5' | -48.8 | NC_004680.1 | + | 1450 | 0.66 | 0.956895 |
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Target: 5'- --gUCCgGGUCaguuUGAGCGGAUCG-Cg -3' miRNA: 3'- aaaAGGaCUAGcu--ACUCGUCUAGCuG- -5' |
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| 18062 | 5' | -48.8 | NC_004680.1 | + | 59235 | 0.69 | 0.867221 |
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Target: 5'- ---aCCUGAUCGAacgcGGGCccgAGAUCGAa -3' miRNA: 3'- aaaaGGACUAGCUa---CUCG---UCUAGCUg -5' |
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| 18062 | 5' | -48.8 | NC_004680.1 | + | 18823 | 0.7 | 0.837798 |
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Target: 5'- ---aCCUGAUCGAagagucgaugccccUGGGCguggugucguGGAUCGGCu -3' miRNA: 3'- aaaaGGACUAGCU--------------ACUCG----------UCUAGCUG- -5' |
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| 18062 | 5' | -48.8 | NC_004680.1 | + | 5347 | 1.09 | 0.004493 |
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Target: 5'- cUUUUCCUGAUCGAUGAGCAGAUCGACg -3' miRNA: 3'- -AAAAGGACUAGCUACUCGUCUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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