miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
18063 3' -58.3 NC_004680.1 + 59381 0.66 0.593749
Target:  5'- cCCGGggGgGUCUGCGCggccccGGaaGGCg -3'
miRNA:   3'- cGGCCuuCaCAGGCGCGa-----CCagUCGa -5'
18063 3' -58.3 NC_004680.1 + 52571 0.67 0.530413
Target:  5'- aGCUGGAuucucgGGUGUUCGUugucgcucauuGCUGGUCcuuuucGGCUa -3'
miRNA:   3'- -CGGCCU------UCACAGGCG-----------CGACCAG------UCGA- -5'
18063 3' -58.3 NC_004680.1 + 40097 0.67 0.530413
Target:  5'- gGCgUGGggGUGacggCC-CGCUGcGUCAGCc -3'
miRNA:   3'- -CG-GCCuuCACa---GGcGCGAC-CAGUCGa -5'
18063 3' -58.3 NC_004680.1 + 35982 0.68 0.469705
Target:  5'- -gCGGAcaaGGUG-CCGCgGCUGGgCGGCUu -3'
miRNA:   3'- cgGCCU---UCACaGGCG-CGACCaGUCGA- -5'
18063 3' -58.3 NC_004680.1 + 3885 0.68 0.466761
Target:  5'- uGUCGGAAGguaagaGcgucgcagcggcacUCCGUGCcGGUCAGCg -3'
miRNA:   3'- -CGGCCUUCa-----C--------------AGGCGCGaCCAGUCGa -5'
18063 3' -58.3 NC_004680.1 + 25627 0.7 0.351149
Target:  5'- cGCCuccuuguGGggGUGggCCGCGUggcggGGUCGGUUu -3'
miRNA:   3'- -CGG-------CCuuCACa-GGCGCGa----CCAGUCGA- -5'
18063 3' -58.3 NC_004680.1 + 8246 0.7 0.327936
Target:  5'- cGCCGGAAGUG-CgGCGC-GcGUaCAGCa -3'
miRNA:   3'- -CGGCCUUCACaGgCGCGaC-CA-GUCGa -5'
18063 3' -58.3 NC_004680.1 + 27846 0.73 0.214787
Target:  5'- gGCCGGAcGGUGguggaCGCGCUGGgcgcgaUCGGCg -3'
miRNA:   3'- -CGGCCU-UCACag---GCGCGACC------AGUCGa -5'
18063 3' -58.3 NC_004680.1 + 31944 0.74 0.203933
Target:  5'- cCCGGAGGaUGUCCGCGgCacGGUCGGUc -3'
miRNA:   3'- cGGCCUUC-ACAGGCGC-Ga-CCAGUCGa -5'
18063 3' -58.3 NC_004680.1 + 5665 1.09 0.000554
Target:  5'- cGCCGGAAGUGUCCGCGCUGGUCAGCUu -3'
miRNA:   3'- -CGGCCUUCACAGGCGCGACCAGUCGA- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.