Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18064 | 5' | -50.3 | NC_004680.1 | + | 6209 | 0.66 | 0.931631 |
Target: 5'- aUAGUua-CCGUCUUgGUUGAAGuCGACu -3' miRNA: 3'- -AUCGacgGGCAGAA-UAACUUCuGCUG- -5' |
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18064 | 5' | -50.3 | NC_004680.1 | + | 54863 | 0.66 | 0.925734 |
Target: 5'- -cGuCUGCCCGgaaccCUgcucGAAGACGGCu -3' miRNA: 3'- auC-GACGGGCa----GAauaaCUUCUGCUG- -5' |
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18064 | 5' | -50.3 | NC_004680.1 | + | 42650 | 0.66 | 0.91303 |
Target: 5'- aGGCgGCCCGcCUgcucGAcGACGGCg -3' miRNA: 3'- aUCGaCGGGCaGAauaaCUuCUGCUG- -5' |
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18064 | 5' | -50.3 | NC_004680.1 | + | 49533 | 0.67 | 0.899124 |
Target: 5'- gGGCggGCCUGUCau-UUGGcuuGGCGACg -3' miRNA: 3'- aUCGa-CGGGCAGaauAACUu--CUGCUG- -5' |
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18064 | 5' | -50.3 | NC_004680.1 | + | 24847 | 0.67 | 0.894722 |
Target: 5'- -cGCUGCCCGgagaucccGAGGugGAUg -3' miRNA: 3'- auCGACGGGCagaauaa-CUUCugCUG- -5' |
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18064 | 5' | -50.3 | NC_004680.1 | + | 33307 | 0.69 | 0.813053 |
Target: 5'- aUGGCgGCCCGguagucGUUGAGGACGcCg -3' miRNA: 3'- -AUCGaCGGGCagaa--UAACUUCUGCuG- -5' |
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18064 | 5' | -50.3 | NC_004680.1 | + | 11897 | 0.69 | 0.80313 |
Target: 5'- cGGCgacUGCCCGggg-GUUGcAGACGGCg -3' miRNA: 3'- aUCG---ACGGGCagaaUAACuUCUGCUG- -5' |
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18064 | 5' | -50.3 | NC_004680.1 | + | 38364 | 0.69 | 0.793011 |
Target: 5'- -cGCUGCCCGUgaggcGUUGGAGcCGAUc -3' miRNA: 3'- auCGACGGGCAgaa--UAACUUCuGCUG- -5' |
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18064 | 5' | -50.3 | NC_004680.1 | + | 5925 | 1.07 | 0.003721 |
Target: 5'- cUAGCUGCCCGUCUUAUUGAAGACGACc -3' miRNA: 3'- -AUCGACGGGCAGAAUAACUUCUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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