Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18065 | 5' | -47.6 | NC_004680.1 | + | 49284 | 0.72 | 0.852989 |
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Target: 5'- -gCACCUGAugGGGcucgauacugccgucGUCACGGacguAGCg -3' miRNA: 3'- caGUGGACUugUCU---------------UAGUGCCau--UCG- -5' |
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| 18065 | 5' | -47.6 | NC_004680.1 | + | 52822 | 0.72 | 0.849418 |
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Target: 5'- --gGCCUGGGCggcgAGAAUCACGGaAAGg -3' miRNA: 3'- cagUGGACUUG----UCUUAGUGCCaUUCg -5' |
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| 18065 | 5' | -47.6 | NC_004680.1 | + | 39360 | 0.72 | 0.839391 |
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Target: 5'- aUCACCUGAgaACAGuguguugguuuugGAUUugGGUuGGCa -3' miRNA: 3'- cAGUGGACU--UGUC-------------UUAGugCCAuUCG- -5' |
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| 18065 | 5' | -47.6 | NC_004680.1 | + | 25037 | 0.75 | 0.692863 |
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Target: 5'- -gCACCUGGGCAGGcagGUCcCGGU-GGCg -3' miRNA: 3'- caGUGGACUUGUCU---UAGuGCCAuUCG- -5' |
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| 18065 | 5' | -47.6 | NC_004680.1 | + | 7014 | 1.15 | 0.00297 |
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Target: 5'- cGUCACCUGAACAGAAUCACGGUAAGCg -3' miRNA: 3'- -CAGUGGACUUGUCUUAGUGCCAUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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