Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18068 | 5' | -51.4 | NC_004680.1 | + | 9779 | 0.66 | 0.921966 |
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Target: 5'- uGUCGUCGUU--GCcUGugGcGGGCCGg -3' miRNA: 3'- -CAGCAGCAGuaUGaGCugUuCCUGGC- -5' |
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| 18068 | 5' | -51.4 | NC_004680.1 | + | 57835 | 0.66 | 0.91454 |
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Target: 5'- cGUCGUCGUCGUAC-CaGGCAccuucaaAGGcuucaucguauugGCCGa -3' miRNA: 3'- -CAGCAGCAGUAUGaG-CUGU-------UCC-------------UGGC- -5' |
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| 18068 | 5' | -51.4 | NC_004680.1 | + | 57471 | 0.67 | 0.902646 |
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Target: 5'- -cCGUCGUCca---CGACGAGGACUu -3' miRNA: 3'- caGCAGCAGuaugaGCUGUUCCUGGc -5' |
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| 18068 | 5' | -51.4 | NC_004680.1 | + | 56418 | 0.67 | 0.902646 |
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Target: 5'- cUCGUCGUCGUcgUCGACGAagauGGugUa -3' miRNA: 3'- cAGCAGCAGUAugAGCUGUU----CCugGc -5' |
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| 18068 | 5' | -51.4 | NC_004680.1 | + | 31674 | 0.67 | 0.888385 |
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Target: 5'- uUgGUCGUCGUugUUGACGgaguGGugCa -3' miRNA: 3'- cAgCAGCAGUAugAGCUGUu---CCugGc -5' |
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| 18068 | 5' | -51.4 | NC_004680.1 | + | 7502 | 1.11 | 0.002504 |
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Target: 5'- cGUCGUCGUCAUACUCGACAAGGACCGg -3' miRNA: 3'- -CAGCAGCAGUAUGAGCUGUUCCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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