Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18072 | 5' | -58.1 | NC_004680.1 | + | 47712 | 0.66 | 0.585275 |
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Target: 5'- ---aGUUCGAu--CUGCUCAGCCCAc -3' miRNA: 3'- acggCAGGCUcauGACGAGUCGGGUc -5' |
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| 18072 | 5' | -58.1 | NC_004680.1 | + | 52017 | 0.66 | 0.585275 |
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Target: 5'- gUGUCGUCC-AGUGCUGCUUucGCgCGc -3' miRNA: 3'- -ACGGCAGGcUCAUGACGAGu-CGgGUc -5' |
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| 18072 | 5' | -58.1 | NC_004680.1 | + | 3833 | 0.66 | 0.563875 |
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Target: 5'- gUGCCGUCgGAgaauguGUAC-GCgCGGCCCGu -3' miRNA: 3'- -ACGGCAGgCU------CAUGaCGaGUCGGGUc -5' |
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| 18072 | 5' | -58.1 | NC_004680.1 | + | 12156 | 0.66 | 0.542689 |
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Target: 5'- -cCCGUCCG-GcGCUGC-C-GCCCAGg -3' miRNA: 3'- acGGCAGGCuCaUGACGaGuCGGGUC- -5' |
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| 18072 | 5' | -58.1 | NC_004680.1 | + | 21929 | 0.66 | 0.532193 |
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Target: 5'- cGCCGaaCGAG-ACguggGaCUCAGCCCAc -3' miRNA: 3'- aCGGCagGCUCaUGa---C-GAGUCGGGUc -5' |
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| 18072 | 5' | -58.1 | NC_004680.1 | + | 55439 | 0.66 | 0.532193 |
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Target: 5'- gGCCGgcgaguUCCGGGgggACacugauggUGCgCAGCCCGGg -3' miRNA: 3'- aCGGC------AGGCUCa--UG--------ACGaGUCGGGUC- -5' |
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| 18072 | 5' | -58.1 | NC_004680.1 | + | 8469 | 1.07 | 0.000689 |
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Target: 5'- cUGCCGUCCGAGUACUGCUCAGCCCAGc -3' miRNA: 3'- -ACGGCAGGCUCAUGACGAGUCGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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