Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18074 | 5' | -54.6 | NC_004680.1 | + | 35916 | 0.69 | 0.554949 |
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Target: 5'- cUUGUCCGCGAucaaucugGCCGCcggggcgcaucaCCCGAACu -3' miRNA: 3'- uAACAGGUGCU--------UGGCGug----------GGGCUUGu -5' |
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| 18074 | 5' | -54.6 | NC_004680.1 | + | 38693 | 0.69 | 0.548461 |
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Target: 5'- --aGUCCAUGAACCGUcUgUCGGGCAu -3' miRNA: 3'- uaaCAGGUGCUUGGCGuGgGGCUUGU- -5' |
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| 18074 | 5' | -54.6 | NC_004680.1 | + | 48502 | 0.7 | 0.527015 |
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Target: 5'- --cGUCCGgGGaaGCCGCugCCCGGuCGg -3' miRNA: 3'- uaaCAGGUgCU--UGGCGugGGGCUuGU- -5' |
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| 18074 | 5' | -54.6 | NC_004680.1 | + | 36998 | 0.7 | 0.505891 |
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Target: 5'- --gGUCCGCGugauCCGuUACCCCGAggGCGc -3' miRNA: 3'- uaaCAGGUGCuu--GGC-GUGGGGCU--UGU- -5' |
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| 18074 | 5' | -54.6 | NC_004680.1 | + | 11786 | 0.73 | 0.353241 |
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Target: 5'- -gUGUCCAUG-ACCGCucuCCCCGGGg- -3' miRNA: 3'- uaACAGGUGCuUGGCGu--GGGGCUUgu -5' |
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| 18074 | 5' | -54.6 | NC_004680.1 | + | 9238 | 1.07 | 0.001672 |
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Target: 5'- gAUUGUCCACGAACCGCACCCCGAACAg -3' miRNA: 3'- -UAACAGGUGCUUGGCGUGGGGCUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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