Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18075 | 3' | -57.9 | NC_004680.1 | + | 33629 | 0.67 | 0.533205 |
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Target: 5'- -gGGCGCCCgucagGaAACCGgcGGCGAUC-CGc -3' miRNA: 3'- uaCUGCGGGa----C-UUGGC--CCGCUAGaGC- -5' |
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| 18075 | 3' | -57.9 | NC_004680.1 | + | 8373 | 0.67 | 0.522819 |
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Target: 5'- -gGugGCCC-GGGCUGGGCuGAgcaguaCUCGg -3' miRNA: 3'- uaCugCGGGaCUUGGCCCG-CUa-----GAGC- -5' |
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| 18075 | 3' | -57.9 | NC_004680.1 | + | 27384 | 0.67 | 0.512513 |
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Target: 5'- uUGGCGUUCUgGAACCacuuGGCGAgguUCUCGg -3' miRNA: 3'- uACUGCGGGA-CUUGGc---CCGCU---AGAGC- -5' |
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| 18075 | 3' | -57.9 | NC_004680.1 | + | 57602 | 0.68 | 0.472187 |
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Target: 5'- uUGAUGCCCUGuucgauCUGGGCGAa---- -3' miRNA: 3'- uACUGCGGGACuu----GGCCCGCUagagc -5' |
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| 18075 | 3' | -57.9 | NC_004680.1 | + | 11066 | 0.69 | 0.424151 |
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Target: 5'- gAUGGCaUCCUGcuGCCGGGCGGcCUUGg -3' miRNA: 3'- -UACUGcGGGACu-UGGCCCGCUaGAGC- -5' |
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| 18075 | 3' | -57.9 | NC_004680.1 | + | 35850 | 0.7 | 0.370553 |
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Target: 5'- gGUGGaGUCCUGAG-CGGGCGGUCggCGa -3' miRNA: 3'- -UACUgCGGGACUUgGCCCGCUAGa-GC- -5' |
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| 18075 | 3' | -57.9 | NC_004680.1 | + | 15879 | 0.74 | 0.215674 |
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Target: 5'- cGUGGCG-CCUGAGCCGGGUGAggacaccggUgUCGa -3' miRNA: 3'- -UACUGCgGGACUUGGCCCGCU---------AgAGC- -5' |
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| 18075 | 3' | -57.9 | NC_004680.1 | + | 9383 | 1.06 | 0.000983 |
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Target: 5'- aAUGACGCCCUGAACCGGGCGAUCUCGu -3' miRNA: 3'- -UACUGCGGGACUUGGCCCGCUAGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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