Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18076 | 3' | -52.7 | NC_004680.1 | + | 55018 | 0.66 | 0.867953 |
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Target: 5'- aUCAugCGGCGCauggCUCGCcgccugaUCUCUg -3' miRNA: 3'- gAGUugGCUGCGa---GAGCGaa-----AGAGGu -5' |
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| 18076 | 3' | -52.7 | NC_004680.1 | + | 39706 | 0.66 | 0.851243 |
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Target: 5'- -cCGugCGGCGCUCccUGCggcgCUCCAu -3' miRNA: 3'- gaGUugGCUGCGAGa-GCGaaa-GAGGU- -5' |
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| 18076 | 3' | -52.7 | NC_004680.1 | + | 39805 | 0.67 | 0.805504 |
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Target: 5'- gCUCccugCGGCGCUCUCuGCggcgCUCCAu -3' miRNA: 3'- -GAGuug-GCUGCGAGAG-CGaaa-GAGGU- -5' |
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| 18076 | 3' | -52.7 | NC_004680.1 | + | 39670 | 0.67 | 0.785829 |
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Target: 5'- -cCAugCGGCGCUCccUGCggcgCUCCAu -3' miRNA: 3'- gaGUugGCUGCGAGa-GCGaaa-GAGGU- -5' |
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| 18076 | 3' | -52.7 | NC_004680.1 | + | 3315 | 0.67 | 0.785829 |
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Target: 5'- cCUCAGCCGACugGUUCgCGCUg--UCCGg -3' miRNA: 3'- -GAGUUGGCUG--CGAGaGCGAaagAGGU- -5' |
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| 18076 | 3' | -52.7 | NC_004680.1 | + | 39766 | 0.67 | 0.785829 |
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Target: 5'- -cCAugCGGCGCUCccUGCggcgCUCCAu -3' miRNA: 3'- gaGUugGCUGCGAGa-GCGaaa-GAGGU- -5' |
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| 18076 | 3' | -52.7 | NC_004680.1 | + | 39850 | 0.67 | 0.785829 |
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Target: 5'- -cCAugCGGCGCUCccUGCggcgCUCCAu -3' miRNA: 3'- gaGUugGCUGCGAGa-GCGaaa-GAGGU- -5' |
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| 18076 | 3' | -52.7 | NC_004680.1 | + | 39910 | 0.7 | 0.612697 |
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Target: 5'- -cCGugCGGCGCUCUCuGCggcgCUCCc -3' miRNA: 3'- gaGUugGCUGCGAGAG-CGaaa-GAGGu -5' |
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| 18076 | 3' | -52.7 | NC_004680.1 | + | 39946 | 0.72 | 0.53565 |
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Target: 5'- -cCAugCGGCGCUCUCuGCggcgCUCCc -3' miRNA: 3'- gaGUugGCUGCGAGAG-CGaaa-GAGGu -5' |
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| 18076 | 3' | -52.7 | NC_004680.1 | + | 53553 | 0.73 | 0.472439 |
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Target: 5'- uCUgGACCGACGCcCUCGUacucCUCCAg -3' miRNA: 3'- -GAgUUGGCUGCGaGAGCGaaa-GAGGU- -5' |
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| 18076 | 3' | -52.7 | NC_004680.1 | + | 9475 | 1.09 | 0.00189 |
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Target: 5'- cCUCAACCGACGCUCUCGCUUUCUCCAa -3' miRNA: 3'- -GAGUUGGCUGCGAGAGCGAAAGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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