Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18078 | 3' | -55.3 | NC_004680.1 | + | 33206 | 0.66 | 0.700302 |
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Target: 5'- gGUgGUGgcGAUGCgGCCCucuuCGACGUc -3' miRNA: 3'- -UAgCACaaCUACGgUGGGuc--GCUGCA- -5' |
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| 18078 | 3' | -55.3 | NC_004680.1 | + | 8539 | 0.67 | 0.656424 |
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Target: 5'- uUUGUG-UGGUGCCugCCGG-GugGg -3' miRNA: 3'- uAGCACaACUACGGugGGUCgCugCa -5' |
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| 18078 | 3' | -55.3 | NC_004680.1 | + | 19228 | 0.67 | 0.64537 |
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Target: 5'- -aCGUGUUGuuucuggugGCCG-CCAGCGACa- -3' miRNA: 3'- uaGCACAACua-------CGGUgGGUCGCUGca -5' |
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| 18078 | 3' | -55.3 | NC_004680.1 | + | 6062 | 0.67 | 0.623237 |
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Target: 5'- -gCGUGUg---GCUGCCUGGCGugGUg -3' miRNA: 3'- uaGCACAacuaCGGUGGGUCGCugCA- -5' |
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| 18078 | 3' | -55.3 | NC_004680.1 | + | 19502 | 0.67 | 0.612177 |
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Target: 5'- gGUgGUGUUGGUGgC-CCgGGUGACGg -3' miRNA: 3'- -UAgCACAACUACgGuGGgUCGCUGCa -5' |
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| 18078 | 3' | -55.3 | NC_004680.1 | + | 5262 | 0.69 | 0.525065 |
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Target: 5'- -aCG-GUaGGUGCCACcacgCCAGCGAUGUg -3' miRNA: 3'- uaGCaCAaCUACGGUG----GGUCGCUGCA- -5' |
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| 18078 | 3' | -55.3 | NC_004680.1 | + | 17287 | 0.72 | 0.360104 |
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Target: 5'- gAUCGUGUgGcgGCCGguacaCCGGCGACGa -3' miRNA: 3'- -UAGCACAaCuaCGGUg----GGUCGCUGCa -5' |
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| 18078 | 3' | -55.3 | NC_004680.1 | + | 10839 | 1.06 | 0.001607 |
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Target: 5'- cAUCGUGUUGAUGCCACCCAGCGACGUg -3' miRNA: 3'- -UAGCACAACUACGGUGGGUCGCUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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