Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18079 | 3' | -56.8 | NC_004680.1 | + | 14511 | 0.7 | 0.416543 |
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Target: 5'- ---cUG-CGGCgACGGCGGCACAGaCGu -3' miRNA: 3'- ugcaACaGUCGgUGUCGCCGUGUC-GC- -5' |
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| 18079 | 3' | -56.8 | NC_004680.1 | + | 19576 | 0.7 | 0.397207 |
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Target: 5'- cACGUauuccauUGUC-GCUgAgGGCGGCGCGGCGg -3' miRNA: 3'- -UGCA-------ACAGuCGG-UgUCGCCGUGUCGC- -5' |
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| 18079 | 3' | -56.8 | NC_004680.1 | + | 22034 | 0.71 | 0.3544 |
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Target: 5'- cGCGacUGUgGGCCACGGCGGC-CAuGCc -3' miRNA: 3'- -UGCa-ACAgUCGGUGUCGCCGuGU-CGc -5' |
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| 18079 | 3' | -56.8 | NC_004680.1 | + | 21360 | 0.75 | 0.198182 |
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Target: 5'- ----cGUCGGCCACguauGGCGGCGcCGGCGg -3' miRNA: 3'- ugcaaCAGUCGGUG----UCGCCGU-GUCGC- -5' |
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| 18079 | 3' | -56.8 | NC_004680.1 | + | 32396 | 0.82 | 0.061415 |
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Target: 5'- gUGUUGUCccugAGCCGCAGCGGuCGCAGCu -3' miRNA: 3'- uGCAACAG----UCGGUGUCGCC-GUGUCGc -5' |
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| 18079 | 3' | -56.8 | NC_004680.1 | + | 10934 | 1.09 | 0.000734 |
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Target: 5'- aACGUUGUCAGCCACAGCGGCACAGCGa -3' miRNA: 3'- -UGCAACAGUCGGUGUCGCCGUGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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