Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18080 | 3' | -58.8 | NC_004680.1 | + | 14970 | 0.66 | 0.573092 |
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Target: 5'- aCCGAUGGGcGcGGuuGGUggcgauuugAUCgAGGcGUCg -3' miRNA: 3'- -GGCUACCC-CuCCggCCA---------UAG-UCC-CAG- -5' |
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| 18080 | 3' | -58.8 | NC_004680.1 | + | 17137 | 0.67 | 0.531546 |
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Target: 5'- aCCGGUGaGGGA-GCCGaUGUUcGGGUUg -3' miRNA: 3'- -GGCUAC-CCCUcCGGCcAUAGuCCCAG- -5' |
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| 18080 | 3' | -58.8 | NC_004680.1 | + | 41189 | 0.68 | 0.471473 |
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Target: 5'- gUCGaAUGGGGcuuGGCCGGauUCcuGGUCa -3' miRNA: 3'- -GGC-UACCCCu--CCGGCCauAGucCCAG- -5' |
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| 18080 | 3' | -58.8 | NC_004680.1 | + | 1839 | 0.69 | 0.371299 |
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Target: 5'- gCCGcGUGGGcGGGCCGGUucccggAUC-GGGUUg -3' miRNA: 3'- -GGC-UACCCcUCCGGCCA------UAGuCCCAG- -5' |
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| 18080 | 3' | -58.8 | NC_004680.1 | + | 56891 | 0.7 | 0.354707 |
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Target: 5'- gCCGGUGGGGuucAGGCUGagggcGUGcugCAGGGUg -3' miRNA: 3'- -GGCUACCCC---UCCGGC-----CAUa--GUCCCAg -5' |
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| 18080 | 3' | -58.8 | NC_004680.1 | + | 25055 | 0.72 | 0.253499 |
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Target: 5'- cCCGGUGGcGGugGGGCCGGUcgggAUC-GGGUa -3' miRNA: 3'- -GGCUACC-CC--UCCGGCCA----UAGuCCCAg -5' |
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| 18080 | 3' | -58.8 | NC_004680.1 | + | 25622 | 0.74 | 0.191669 |
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Target: 5'- cCUuGUGGGGguGGGCCGcGUggCGGGGUCg -3' miRNA: 3'- -GGcUACCCC--UCCGGC-CAuaGUCCCAG- -5' |
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| 18080 | 3' | -58.8 | NC_004680.1 | + | 11085 | 1.11 | 0.00037 |
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Target: 5'- cCCGAUGGGGAGGCCGGUAUCAGGGUCg -3' miRNA: 3'- -GGCUACCCCUCCGGCCAUAGUCCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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