Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18081 | 3' | -57.9 | NC_004680.1 | + | 25724 | 0.66 | 0.540596 |
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Target: 5'- cGCAGUGUUUgggUGCGGCGUcggcgacGgCGGGGAUg -3' miRNA: 3'- -CGUCGCAGG---ACGCCGUA-------CgGUCCUUA- -5' |
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| 18081 | 3' | -57.9 | NC_004680.1 | + | 8928 | 0.67 | 0.510052 |
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Target: 5'- aGCAGaucaacgGUCgUGCuGCugGUGCCAGGGAUg -3' miRNA: 3'- -CGUCg------CAGgACGcCG--UACGGUCCUUA- -5' |
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| 18081 | 3' | -57.9 | NC_004680.1 | + | 39655 | 0.67 | 0.469171 |
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Target: 5'- uGCGGCGcUCCaUGCGGCGcuccaUGCaaauGGAAc -3' miRNA: 3'- -CGUCGC-AGG-ACGCCGU-----ACGgu--CCUUa -5' |
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| 18081 | 3' | -57.9 | NC_004680.1 | + | 9720 | 0.68 | 0.449367 |
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Target: 5'- cGCGGCGaauggUgUGuCGGCggGCCGGGggUu -3' miRNA: 3'- -CGUCGCa----GgAC-GCCGuaCGGUCCuuA- -5' |
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| 18081 | 3' | -57.9 | NC_004680.1 | + | 21624 | 0.68 | 0.43964 |
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Target: 5'- uGCAgGCGUUCU-CGGCcgggGCCGGGGGg -3' miRNA: 3'- -CGU-CGCAGGAcGCCGua--CGGUCCUUa -5' |
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| 18081 | 3' | -57.9 | NC_004680.1 | + | 39030 | 0.68 | 0.411197 |
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Target: 5'- cGCGGCGaguUCCUccgcaGUGGCGgUGuCCAGGAAc -3' miRNA: 3'- -CGUCGC---AGGA-----CGCCGU-AC-GGUCCUUa -5' |
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| 18081 | 3' | -57.9 | NC_004680.1 | + | 41431 | 0.69 | 0.375103 |
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Target: 5'- uCAGCGUgUggUGCGGCGagGCCGGGGc- -3' miRNA: 3'- cGUCGCAgG--ACGCCGUa-CGGUCCUua -5' |
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| 18081 | 3' | -57.9 | NC_004680.1 | + | 57249 | 0.69 | 0.357878 |
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Target: 5'- -gGGCGUUCUcgGCGGC--GCCGGGGAa -3' miRNA: 3'- cgUCGCAGGA--CGCCGuaCGGUCCUUa -5' |
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| 18081 | 3' | -57.9 | NC_004680.1 | + | 11185 | 0.72 | 0.240458 |
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Target: 5'- cGCGGCGUUCUGCuugGGC--GCCGGGGc- -3' miRNA: 3'- -CGUCGCAGGACG---CCGuaCGGUCCUua -5' |
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| 18081 | 3' | -57.9 | NC_004680.1 | + | 11349 | 1.07 | 0.000711 |
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Target: 5'- gGCAGCGUCCUGCGGCAUGCCAGGAAUc -3' miRNA: 3'- -CGUCGCAGGACGCCGUACGGUCCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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