Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18082 | 5' | -53.9 | NC_004680.1 | + | 45243 | 0.67 | 0.685914 |
|
Target: 5'- ---cGCCGAGAccacggGGCCUGCGAUggugUGCa -3' miRNA: 3'- uaucCGGCUUUa-----CCGGACGUUG----ACGg -5' |
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| 18082 | 5' | -53.9 | NC_004680.1 | + | 51940 | 0.68 | 0.61849 |
|
Target: 5'- -aGGGCUGAAAUGaCCUcugGCGugUGCg -3' miRNA: 3'- uaUCCGGCUUUACcGGA---CGUugACGg -5' |
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| 18082 | 5' | -53.9 | NC_004680.1 | + | 55132 | 0.72 | 0.403904 |
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Target: 5'- gGUGGGUCGAGGUGGCgUgacacagagaucagGCGGCgaGCCa -3' miRNA: 3'- -UAUCCGGCUUUACCGgA--------------CGUUGa-CGG- -5' |
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| 18082 | 5' | -53.9 | NC_004680.1 | + | 58439 | 0.68 | 0.663533 |
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Target: 5'- -aAGGUCGGugugGAUGGCUgUGCcGACUGCg -3' miRNA: 3'- uaUCCGGCU----UUACCGG-ACG-UUGACGg -5' |
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| 18082 | 5' | -53.9 | NC_004680.1 | + | 58532 | 0.66 | 0.729932 |
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Target: 5'- uGUAGGCgaUGguGUGGCCUugGUGGCgUGCCc -3' miRNA: 3'- -UAUCCG--GCuuUACCGGA--CGUUG-ACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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