Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18082 | 5' | -53.9 | NC_004680.1 | + | 10004 | 0.71 | 0.476401 |
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Target: 5'- -gGGGaCCGGucgGGCCgGCAucgacgucGCUGCCg -3' miRNA: 3'- uaUCC-GGCUuuaCCGGaCGU--------UGACGG- -5' |
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| 18082 | 5' | -53.9 | NC_004680.1 | + | 21560 | 0.72 | 0.406745 |
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Target: 5'- -cGGGCCGG--UGGCCUcaucuggcgaGCAAC-GCCg -3' miRNA: 3'- uaUCCGGCUuuACCGGA----------CGUUGaCGG- -5' |
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| 18082 | 5' | -53.9 | NC_004680.1 | + | 55132 | 0.72 | 0.403904 |
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Target: 5'- gGUGGGUCGAGGUGGCgUgacacagagaucagGCGGCgaGCCa -3' miRNA: 3'- -UAUCCGGCUUUACCGgA--------------CGUUGa-CGG- -5' |
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| 18082 | 5' | -53.9 | NC_004680.1 | + | 21699 | 0.74 | 0.303367 |
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Target: 5'- --cGGCCGAGAacGCCUGCAGC-GUCa -3' miRNA: 3'- uauCCGGCUUUacCGGACGUUGaCGG- -5' |
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| 18082 | 5' | -53.9 | NC_004680.1 | + | 11724 | 1.09 | 0.001174 |
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Target: 5'- uAUAGGCCGAAAUGGCCUGCAACUGCCc -3' miRNA: 3'- -UAUCCGGCUUUACCGGACGUUGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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