Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18084 | 5' | -54.6 | NC_004680.1 | + | 51206 | 0.66 | 0.798601 |
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Target: 5'- -----aGCCGGUCGAAaucCGUUCCGu -3' miRNA: 3'- uccuuaCGGCCAGCUUcuaGCGAGGC- -5' |
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| 18084 | 5' | -54.6 | NC_004680.1 | + | 25965 | 0.66 | 0.78891 |
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Target: 5'- cAGGAuaagcuccUGCCGG---GAGcUCGCUCCGg -3' miRNA: 3'- -UCCUu-------ACGGCCagcUUCuAGCGAGGC- -5' |
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| 18084 | 5' | -54.6 | NC_004680.1 | + | 44092 | 0.69 | 0.619437 |
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Target: 5'- gAGGAAUGCCGG---GAGuauUCGC-CCGa -3' miRNA: 3'- -UCCUUACGGCCagcUUCu--AGCGaGGC- -5' |
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| 18084 | 5' | -54.6 | NC_004680.1 | + | 10823 | 0.7 | 0.565041 |
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Target: 5'- gGGGAucaGUGCagcacuGUCGAGGAUCGCUgugCCGc -3' miRNA: 3'- -UCCU---UACGgc----CAGCUUCUAGCGA---GGC- -5' |
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| 18084 | 5' | -54.6 | NC_004680.1 | + | 11080 | 0.74 | 0.32669 |
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Target: 5'- uGGGGAgGCCGGUaucAGGGUCGCUgCCGa -3' miRNA: 3'- -UCCUUaCGGCCAgc-UUCUAGCGA-GGC- -5' |
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| 18084 | 5' | -54.6 | NC_004680.1 | + | 12100 | 1.08 | 0.001557 |
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Target: 5'- uAGGAAUGCCGGUCGAAGAUCGCUCCGu -3' miRNA: 3'- -UCCUUACGGCCAGCUUCUAGCGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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