Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18085 | 3' | -55.9 | NC_004680.1 | + | 10578 | 0.66 | 0.684545 |
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Target: 5'- -----cUCGUUGGCUCuuCGGUGGGCu -3' miRNA: 3'- aacacaAGCAGCCGGGugGCCACUUG- -5' |
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| 18085 | 3' | -55.9 | NC_004680.1 | + | 48208 | 0.66 | 0.662699 |
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Target: 5'- gUGgaagGUUCgGUUGGCUCGCCGGcGcGACg -3' miRNA: 3'- aACa---CAAG-CAGCCGGGUGGCCaC-UUG- -5' |
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| 18085 | 3' | -55.9 | NC_004680.1 | + | 21253 | 0.67 | 0.607766 |
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Target: 5'- -aGUG-UCGUCGGCCgggGCUGGUGucgguAGCg -3' miRNA: 3'- aaCACaAGCAGCCGGg--UGGCCAC-----UUG- -5' |
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| 18085 | 3' | -55.9 | NC_004680.1 | + | 26087 | 0.67 | 0.607766 |
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Target: 5'- aUGUGcagUCccgCGGcCCCGCCGGcugGAACg -3' miRNA: 3'- aACACa--AGca-GCC-GGGUGGCCa--CUUG- -5' |
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| 18085 | 3' | -55.9 | NC_004680.1 | + | 47931 | 0.67 | 0.607766 |
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Target: 5'- -gGUGUUUGUgCGGCCgAa-GGUGAAUg -3' miRNA: 3'- aaCACAAGCA-GCCGGgUggCCACUUG- -5' |
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| 18085 | 3' | -55.9 | NC_004680.1 | + | 19505 | 0.69 | 0.479926 |
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Target: 5'- -gGUGUUgGUggcccgggugaCGGCgCCGCCGGUGGuaACa -3' miRNA: 3'- aaCACAAgCA-----------GCCG-GGUGGCCACU--UG- -5' |
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| 18085 | 3' | -55.9 | NC_004680.1 | + | 12579 | 0.95 | 0.00879 |
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Target: 5'- gUUGUGUUCGUCGG-CCACCGGUGAACa -3' miRNA: 3'- -AACACAAGCAGCCgGGUGGCCACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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