Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18093 | 3' | -55.1 | NC_004680.1 | + | 19591 | 0.66 | 0.723706 |
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Target: 5'- cGCUGaGGGcggcGCGGCGGGUaCCGCu- -3' miRNA: 3'- -CGACgCUCuua-CGCUGCCCA-GGUGuu -5' |
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| 18093 | 3' | -55.1 | NC_004680.1 | + | 54480 | 0.66 | 0.712976 |
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Target: 5'- aGCUGCauGAGUuCG-UGGGUCCACAu -3' miRNA: 3'- -CGACGcuCUUAcGCuGCCCAGGUGUu -5' |
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| 18093 | 3' | -55.1 | NC_004680.1 | + | 24837 | 0.66 | 0.691288 |
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Target: 5'- uCUGCGGGAG-GCGcCGGGgaCUACAAc -3' miRNA: 3'- cGACGCUCUUaCGCuGCCCa-GGUGUU- -5' |
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| 18093 | 3' | -55.1 | NC_004680.1 | + | 49905 | 0.67 | 0.647309 |
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Target: 5'- aCUGCcAGggUGCGcgGCGGGgugaaugugauuUCCGCGAc -3' miRNA: 3'- cGACGcUCuuACGC--UGCCC------------AGGUGUU- -5' |
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| 18093 | 3' | -55.1 | NC_004680.1 | + | 52149 | 0.68 | 0.592087 |
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Target: 5'- --aGCGGuccGUGCGACGGGUCUucGCAc -3' miRNA: 3'- cgaCGCUcu-UACGCUGCCCAGG--UGUu -5' |
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| 18093 | 3' | -55.1 | NC_004680.1 | + | 17253 | 0.69 | 0.51641 |
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Target: 5'- --gGCGGGAccUGCGGCGGGUUCGuCGGa -3' miRNA: 3'- cgaCGCUCUu-ACGCUGCCCAGGU-GUU- -5' |
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| 18093 | 3' | -55.1 | NC_004680.1 | + | 9690 | 0.69 | 0.51641 |
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Target: 5'- gGCUGCGGGuucucgcauuGUG-GAUGGGUUCGCGg -3' miRNA: 3'- -CGACGCUCu---------UACgCUGCCCAGGUGUu -5' |
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| 18093 | 3' | -55.1 | NC_004680.1 | + | 17006 | 0.7 | 0.474914 |
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Target: 5'- --cGCGAcug-GgGACGGGUCCACAu -3' miRNA: 3'- cgaCGCUcuuaCgCUGCCCAGGUGUu -5' |
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| 18093 | 3' | -55.1 | NC_004680.1 | + | 1655 | 0.71 | 0.416004 |
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Target: 5'- gGCUGUcguggucacuGAGAuUGCGACGGGcCgGCAGa -3' miRNA: 3'- -CGACG----------CUCUuACGCUGCCCaGgUGUU- -5' |
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| 18093 | 3' | -55.1 | NC_004680.1 | + | 18028 | 1.08 | 0.001202 |
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Target: 5'- cGCUGCGAGAAUGCGACGGGUCCACAAc -3' miRNA: 3'- -CGACGCUCUUACGCUGCCCAGGUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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