Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18093 | 5' | -59.5 | NC_004680.1 | + | 13969 | 0.66 | 0.575605 |
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Target: 5'- uGUUCaaCGCCCGCUgcugguGGCUCCgcugaUCGGGGa -3' miRNA: 3'- gCAAGa-GCGGGUGG------CUGGGG-----AGUCCC- -5' |
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| 18093 | 5' | -59.5 | NC_004680.1 | + | 51432 | 0.66 | 0.534508 |
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Target: 5'- -aUUC-CGCCCACCccuGACCCaccaAGGGc -3' miRNA: 3'- gcAAGaGCGGGUGG---CUGGGgag-UCCC- -5' |
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| 18093 | 5' | -59.5 | NC_004680.1 | + | 21471 | 0.67 | 0.494496 |
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Target: 5'- gCGUUgCUCGCcagaugagggCCACCGGCCCgggCuGGGu -3' miRNA: 3'- -GCAA-GAGCG----------GGUGGCUGGGga-GuCCC- -5' |
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| 18093 | 5' | -59.5 | NC_004680.1 | + | 10078 | 0.67 | 0.494496 |
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Target: 5'- ------gGCCCgACCGGuCCCCUCAaGGGu -3' miRNA: 3'- gcaagagCGGG-UGGCU-GGGGAGU-CCC- -5' |
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| 18093 | 5' | -59.5 | NC_004680.1 | + | 23678 | 0.67 | 0.484703 |
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Target: 5'- aGggCUgCGCCCGCaaACCCCUCGGc- -3' miRNA: 3'- gCaaGA-GCGGGUGgcUGGGGAGUCcc -5' |
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| 18093 | 5' | -59.5 | NC_004680.1 | + | 35764 | 0.68 | 0.455904 |
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Target: 5'- ----gUCGCCgACCG-CCCgCUCAGGa -3' miRNA: 3'- gcaagAGCGGgUGGCuGGG-GAGUCCc -5' |
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| 18093 | 5' | -59.5 | NC_004680.1 | + | 31844 | 0.68 | 0.437226 |
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Target: 5'- --cUC-CGCCaCACCGACCCCggcugagaCAGGc -3' miRNA: 3'- gcaAGaGCGG-GUGGCUGGGGa-------GUCCc -5' |
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| 18093 | 5' | -59.5 | NC_004680.1 | + | 4316 | 0.7 | 0.332855 |
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Target: 5'- aGUUCUCagGCCCcagaagcauccagcGCUGAcCCCCUCGGcGGu -3' miRNA: 3'- gCAAGAG--CGGG--------------UGGCU-GGGGAGUC-CC- -5' |
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| 18093 | 5' | -59.5 | NC_004680.1 | + | 36573 | 0.71 | 0.270786 |
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Target: 5'- cCGUcaUCGCCaACa-ACCCCUCAGGGg -3' miRNA: 3'- -GCAagAGCGGgUGgcUGGGGAGUCCC- -5' |
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| 18093 | 5' | -59.5 | NC_004680.1 | + | 17993 | 1.1 | 0.000434 |
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Target: 5'- gCGUUCUCGCCCACCGACCCCUCAGGGa -3' miRNA: 3'- -GCAAGAGCGGGUGGCUGGGGAGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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