Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18096 | 3' | -59.6 | NC_004680.1 | + | 39499 | 0.66 | 0.472187 |
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Target: 5'- gAGGCUGGCGCGgua-GCGAUC-CgUGa -3' miRNA: 3'- -UUCGACCGCGCgcugCGCUAGaGgAC- -5' |
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| 18096 | 3' | -59.6 | NC_004680.1 | + | 2594 | 0.69 | 0.327206 |
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Target: 5'- -uGCUGGCGuCGUGAUgacgguacgGCGAcgaucacagcugauUCUCCUGa -3' miRNA: 3'- uuCGACCGC-GCGCUG---------CGCU--------------AGAGGAC- -5' |
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| 18096 | 3' | -59.6 | NC_004680.1 | + | 5219 | 0.7 | 0.284909 |
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Target: 5'- -cGCUGGCcggcagaccucgGCG-GACGCGAUUUCCg- -3' miRNA: 3'- uuCGACCG------------CGCgCUGCGCUAGAGGac -5' |
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| 18096 | 3' | -59.6 | NC_004680.1 | + | 9566 | 0.76 | 0.101411 |
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Target: 5'- cGGCUGGgGCGCGugGCGGUucggCUgCUGg -3' miRNA: 3'- uUCGACCgCGCGCugCGCUA----GAgGAC- -5' |
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| 18096 | 3' | -59.6 | NC_004680.1 | + | 18735 | 1.06 | 0.000597 |
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Target: 5'- cAAGCUGGCGCGCGACGCGAUCUCCUGa -3' miRNA: 3'- -UUCGACCGCGCGCUGCGCUAGAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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