Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18097 | 5' | -52.5 | NC_004680.1 | + | 59187 | 0.66 | 0.871247 |
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Target: 5'- uGGUCGGCguggaguucGCCGGUGu-UGGGUUGUg -3' miRNA: 3'- uCCAGUUG---------CGGCUACucACUCAACGa -5' |
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| 18097 | 5' | -52.5 | NC_004680.1 | + | 46237 | 0.66 | 0.845929 |
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Target: 5'- cAGGauUCGAgGCCGGucUGuGUGAGgugUGCg -3' miRNA: 3'- -UCC--AGUUgCGGCU--ACuCACUCa--ACGa -5' |
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| 18097 | 5' | -52.5 | NC_004680.1 | + | 56899 | 0.67 | 0.808947 |
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Target: 5'- aGGG-CggUGCCGGUGGGguucaggcUGAGggcgUGCUg -3' miRNA: 3'- -UCCaGuuGCGGCUACUC--------ACUCa---ACGA- -5' |
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| 18097 | 5' | -52.5 | NC_004680.1 | + | 14323 | 0.67 | 0.789254 |
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Target: 5'- cGGUCccGGCGCgGAUGAGUaugugGAGUuugguccgcUGCUg -3' miRNA: 3'- uCCAG--UUGCGgCUACUCA-----CUCA---------ACGA- -5' |
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| 18097 | 5' | -52.5 | NC_004680.1 | + | 33380 | 0.67 | 0.788251 |
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Target: 5'- cAGGUCucgcacAUGCUGcgGGGUGAGUuugucgaUGCg -3' miRNA: 3'- -UCCAGu-----UGCGGCuaCUCACUCA-------ACGa -5' |
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| 18097 | 5' | -52.5 | NC_004680.1 | + | 27002 | 0.7 | 0.648922 |
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Target: 5'- aGGGUU--CGCCGuUGGGUccGGGUUGCUg -3' miRNA: 3'- -UCCAGuuGCGGCuACUCA--CUCAACGA- -5' |
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| 18097 | 5' | -52.5 | NC_004680.1 | + | 55238 | 0.7 | 0.648922 |
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Target: 5'- cGGGUCGugGaCGGUGAGUGAGc---- -3' miRNA: 3'- -UCCAGUugCgGCUACUCACUCaacga -5' |
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| 18097 | 5' | -52.5 | NC_004680.1 | + | 53003 | 0.74 | 0.403604 |
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Target: 5'- gAGGUCAACGCCGAUGAauugcccagcgauGUcGGGUcgguugUGCa -3' miRNA: 3'- -UCCAGUUGCGGCUACU-------------CA-CUCA------ACGa -5' |
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| 18097 | 5' | -52.5 | NC_004680.1 | + | 18885 | 1.08 | 0.002468 |
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Target: 5'- cAGGUCAACGCCGAUGAGUGAGUUGCUc -3' miRNA: 3'- -UCCAGUUGCGGCUACUCACUCAACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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