Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
181 | 3' | -55.9 | AC_000007.1 | + | 26920 | 0.66 | 0.551815 |
Target: 5'- uACGgGUUCGUuggGCGCcaGACGCAgCGCUc -3' miRNA: 3'- -UGCaUAAGCG---CGCG--UUGCGUgGCGGu -5' |
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181 | 3' | -55.9 | AC_000007.1 | + | 25377 | 0.66 | 0.551815 |
Target: 5'- aACGcagUCGCGgacguaguCGCGGCGCGCCucGCCc -3' miRNA: 3'- -UGCauaAGCGC--------GCGUUGCGUGG--CGGu -5' |
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181 | 3' | -55.9 | AC_000007.1 | + | 10517 | 0.66 | 0.574106 |
Target: 5'- cGCGacuUUcCGCGCGCcuccAC-CACCGCCGc -3' miRNA: 3'- -UGCau-AA-GCGCGCGu---UGcGUGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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