Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18102 | 5' | -54.7 | NC_004680.1 | + | 25161 | 0.66 | 0.808454 |
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Target: 5'- aCCaCCUUGUCGugGcGCagcGGAAaaggugcGGACGg -3' miRNA: 3'- -GG-GGAACAGCugC-CGga-CCUU-------CUUGC- -5' |
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| 18102 | 5' | -54.7 | NC_004680.1 | + | 44405 | 0.67 | 0.740324 |
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Target: 5'- aCUgCUgggGUUGGCGGCUggcgGGGAGuACGg -3' miRNA: 3'- -GGgGAa--CAGCUGCCGGa---CCUUCuUGC- -5' |
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| 18102 | 5' | -54.7 | NC_004680.1 | + | 11889 | 0.67 | 0.729946 |
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Target: 5'- gCCCggggGUUgcaGACGGCgCcccgGGGAGAGCGg -3' miRNA: 3'- gGGGaa--CAG---CUGCCG-Ga---CCUUCUUGC- -5' |
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| 18102 | 5' | -54.7 | NC_004680.1 | + | 47748 | 0.68 | 0.655249 |
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Target: 5'- aCCCCggccaacaCGuCGGCCUGGuuGAACu -3' miRNA: 3'- -GGGGaaca----GCuGCCGGACCuuCUUGc -5' |
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| 18102 | 5' | -54.7 | NC_004680.1 | + | 4347 | 0.7 | 0.568767 |
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Target: 5'- aCCCCc--UCGGCGGUCUGGAu-AACGc -3' miRNA: 3'- -GGGGaacAGCUGCCGGACCUucUUGC- -5' |
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| 18102 | 5' | -54.7 | NC_004680.1 | + | 42270 | 0.71 | 0.505738 |
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Target: 5'- cCCCCgcccUGUCuACGGagauCCUGGAGGAAgCGg -3' miRNA: 3'- -GGGGa---ACAGcUGCC----GGACCUUCUU-GC- -5' |
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| 18102 | 5' | -54.7 | NC_004680.1 | + | 13484 | 0.72 | 0.436303 |
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Target: 5'- gCCCCaUGUCGugGGUgaGGAAGuuCa -3' miRNA: 3'- -GGGGaACAGCugCCGgaCCUUCuuGc -5' |
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| 18102 | 5' | -54.7 | NC_004680.1 | + | 20456 | 1.1 | 0.001205 |
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Target: 5'- aCCCCUUGUCGACGGCCUGGAAGAACGc -3' miRNA: 3'- -GGGGAACAGCUGCCGGACCUUCUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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