Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18104 | 5' | -53.8 | NC_004680.1 | + | 21432 | 0.65 | 0.781217 |
Target: 5'- gGGGCCUggcgauuuuccgcaUGGAGaaCGCAGgcuccGGCGUUGc -3' miRNA: 3'- -UCCGGA--------------ACUUCaaGUGUCa----CCGCGAU- -5' |
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18104 | 5' | -53.8 | NC_004680.1 | + | 21311 | 0.7 | 0.530514 |
Target: 5'- gGGGCCacGguGUUCGCGGUGGUGg-- -3' miRNA: 3'- -UCCGGaaCuuCAAGUGUCACCGCgau -5' |
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18104 | 5' | -53.8 | NC_004680.1 | + | 33652 | 0.71 | 0.466726 |
Target: 5'- cAGGCCgaGGAGUUCcccACGGcgGGCGCc- -3' miRNA: 3'- -UCCGGaaCUUCAAG---UGUCa-CCGCGau -5' |
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18104 | 5' | -53.8 | NC_004680.1 | + | 21365 | 1.07 | 0.001662 |
Target: 5'- gAGGCCUUGAAGUUCACAGUGGCGCUAc -3' miRNA: 3'- -UCCGGAACUUCAAGUGUCACCGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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