Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18106 | 3' | -58.4 | NC_004680.1 | + | 53271 | 0.66 | 0.542689 |
Target: 5'- uAAAUUUCGGCGUGGagCUggcaGAACgCCCGa -3' miRNA: 3'- -UUUGAAGCCGCGCCg-GAg---CUUG-GGGU- -5' |
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18106 | 3' | -58.4 | NC_004680.1 | + | 29112 | 0.66 | 0.532194 |
Target: 5'- ----aUCGGCGCGgaacGCCgCGGcaACCCCGg -3' miRNA: 3'- uuugaAGCCGCGC----CGGaGCU--UGGGGU- -5' |
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18106 | 3' | -58.4 | NC_004680.1 | + | 19063 | 0.67 | 0.461118 |
Target: 5'- uGAACUccccCGGCGCGGCCgugccaCCCAa -3' miRNA: 3'- -UUUGAa---GCCGCGCCGGagcuugGGGU- -5' |
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18106 | 3' | -58.4 | NC_004680.1 | + | 12419 | 0.67 | 0.451368 |
Target: 5'- gGGACUcccCGGCGCGGCaCcCGAagcggcggucGCCCCc -3' miRNA: 3'- -UUUGAa--GCCGCGCCG-GaGCU----------UGGGGu -5' |
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18106 | 3' | -58.4 | NC_004680.1 | + | 11076 | 0.69 | 0.386489 |
Target: 5'- -uGCUgcCGG-GCGGCCUUGGuggcgGCCCCGg -3' miRNA: 3'- uuUGAa-GCCgCGCCGGAGCU-----UGGGGU- -5' |
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18106 | 3' | -58.4 | NC_004680.1 | + | 25602 | 0.71 | 0.283398 |
Target: 5'- uGGCggggUCGGUuucucaGGCCUUGAGCCCCGc -3' miRNA: 3'- uUUGa---AGCCGcg----CCGGAGCUUGGGGU- -5' |
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18106 | 3' | -58.4 | NC_004680.1 | + | 22277 | 1.06 | 0.000799 |
Target: 5'- cAAACUUCGGCGCGGCCUCGAACCCCAg -3' miRNA: 3'- -UUUGAAGCCGCGCCGGAGCUUGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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