Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18112 | 5' | -51.9 | NC_004680.1 | + | 16570 | 0.66 | 0.898053 |
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Target: 5'- gGAGCGuGCCCua-AUGUCGUgGCCu -3' miRNA: 3'- gUUUGUcCGGGuagUACAGCAaCGGc -5' |
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| 18112 | 5' | -51.9 | NC_004680.1 | + | 12265 | 0.66 | 0.898053 |
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Target: 5'- aCGAACuGGCCCGcccaaggcgcUgGUGUUGgugUGCCc -3' miRNA: 3'- -GUUUGuCCGGGU----------AgUACAGCa--ACGGc -5' |
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| 18112 | 5' | -51.9 | NC_004680.1 | + | 27037 | 0.66 | 0.883245 |
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Target: 5'- -uGGCAGGgUCAUCAUGuuuUCGggugUGCCc -3' miRNA: 3'- guUUGUCCgGGUAGUAC---AGCa---ACGGc -5' |
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| 18112 | 5' | -51.9 | NC_004680.1 | + | 20955 | 0.67 | 0.850451 |
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Target: 5'- -uGACAGGCCCAUg--G-CGUUGUCc -3' miRNA: 3'- guUUGUCCGGGUAguaCaGCAACGGc -5' |
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| 18112 | 5' | -51.9 | NC_004680.1 | + | 11080 | 0.67 | 0.823314 |
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Target: 5'- uGGGgAGGCCggUAUCAggGUCGcUGCCGa -3' miRNA: 3'- gUUUgUCCGG--GUAGUa-CAGCaACGGC- -5' |
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| 18112 | 5' | -51.9 | NC_004680.1 | + | 9896 | 0.68 | 0.774028 |
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Target: 5'- -cGACAGGCaUCAUCGg--CGUUGCUGc -3' miRNA: 3'- guUUGUCCG-GGUAGUacaGCAACGGC- -5' |
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| 18112 | 5' | -51.9 | NC_004680.1 | + | 8855 | 0.7 | 0.698934 |
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Target: 5'- -cGGCAGGaCCAUC---UCGUUGCCGu -3' miRNA: 3'- guUUGUCCgGGUAGuacAGCAACGGC- -5' |
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| 18112 | 5' | -51.9 | NC_004680.1 | + | 20536 | 0.75 | 0.389827 |
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Target: 5'- -cAGCAuguGGCCCAUCGUGaUCGcggUGCCGu -3' miRNA: 3'- guUUGU---CCGGGUAGUAC-AGCa--ACGGC- -5' |
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| 18112 | 5' | -51.9 | NC_004680.1 | + | 23488 | 1.09 | 0.002187 |
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Target: 5'- uCAAACAGGCCCAUCAUGUCGUUGCCGa -3' miRNA: 3'- -GUUUGUCCGGGUAGUACAGCAACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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