Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18121 | 5' | -51.4 | NC_004680.1 | + | 46284 | 0.66 | 0.904594 |
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Target: 5'- uGCG-UGUAcCGGGUCaUGUca-UCCGCa -3' miRNA: 3'- -CGCaACAUaGCCCGG-ACGuaaAGGCG- -5' |
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| 18121 | 5' | -51.4 | NC_004680.1 | + | 24924 | 0.66 | 0.897621 |
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Target: 5'- aGCGUUGUAgucccCGGcGCCUcccGCAgaUCCa- -3' miRNA: 3'- -CGCAACAUa----GCC-CGGA---CGUaaAGGcg -5' |
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| 18121 | 5' | -51.4 | NC_004680.1 | + | 59207 | 0.67 | 0.858702 |
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Target: 5'- cGCGUg--AUCGGGgUUGUAUguggUCgGCg -3' miRNA: 3'- -CGCAacaUAGCCCgGACGUAa---AGgCG- -5' |
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| 18121 | 5' | -51.4 | NC_004680.1 | + | 49527 | 0.68 | 0.841365 |
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Target: 5'- gGCGgUGgg-CGGGCCUGUcaUUUgGCu -3' miRNA: 3'- -CGCaACauaGCCCGGACGuaAAGgCG- -5' |
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| 18121 | 5' | -51.4 | NC_004680.1 | + | 42356 | 0.69 | 0.804032 |
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Target: 5'- gGUGUUGgg-CaGGCCacgaucucggcGCAUUUCCGCg -3' miRNA: 3'- -CGCAACauaGcCCGGa----------CGUAAAGGCG- -5' |
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| 18121 | 5' | -51.4 | NC_004680.1 | + | 7772 | 0.71 | 0.665986 |
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Target: 5'- -gGUUGUcgCGGaugaacaccGCCUGCAgg-CCGCc -3' miRNA: 3'- cgCAACAuaGCC---------CGGACGUaaaGGCG- -5' |
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| 18121 | 5' | -51.4 | NC_004680.1 | + | 20742 | 0.72 | 0.621181 |
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Target: 5'- aCGUcuUGgccgccgAUCGGGCCUGU---UCCGCc -3' miRNA: 3'- cGCA--ACa------UAGCCCGGACGuaaAGGCG- -5' |
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| 18121 | 5' | -51.4 | NC_004680.1 | + | 28956 | 0.73 | 0.565463 |
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Target: 5'- aCGUUGUAguccacgugCGGGCCggUGCGUUU-CGCg -3' miRNA: 3'- cGCAACAUa--------GCCCGG--ACGUAAAgGCG- -5' |
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| 18121 | 5' | -51.4 | NC_004680.1 | + | 28166 | 1.16 | 0.000989 |
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Target: 5'- cGCGUUGUAUCGGGCCUGCAUUUCCGCg -3' miRNA: 3'- -CGCAACAUAGCCCGGACGUAAAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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