Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18129 | 3' | -59 | NC_004680.1 | + | 9968 | 0.66 | 0.542726 |
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Target: 5'- cGAugAUgCCU-G-UCGCGGCGGCCGUg -3' miRNA: 3'- aCU--UAgGGAgCgAGCGCCGUCGGCAg -5' |
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| 18129 | 3' | -59 | NC_004680.1 | + | 37058 | 0.66 | 0.521968 |
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Target: 5'- cUGggUCCC-CaCUCccgGCuGGCAGCCGcCa -3' miRNA: 3'- -ACuuAGGGaGcGAG---CG-CCGUCGGCaG- -5' |
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| 18129 | 3' | -59 | NC_004680.1 | + | 52177 | 0.66 | 0.521968 |
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Target: 5'- cGAAgCCCUCGC-CGCagagcugcgccGGCAaGCCGa- -3' miRNA: 3'- aCUUaGGGAGCGaGCG-----------CCGU-CGGCag -5' |
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| 18129 | 3' | -59 | NC_004680.1 | + | 51229 | 0.66 | 0.501525 |
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Target: 5'- gGAcgCCCUCaGCuUUGCcccGCAGCCGgUCg -3' miRNA: 3'- aCUuaGGGAG-CG-AGCGc--CGUCGGC-AG- -5' |
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| 18129 | 3' | -59 | NC_004680.1 | + | 34786 | 0.67 | 0.481441 |
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Target: 5'- gUGAugGUCaCCgCGCUCGCuGuGCucGCCGUCg -3' miRNA: 3'- -ACU--UAG-GGaGCGAGCG-C-CGu-CGGCAG- -5' |
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| 18129 | 3' | -59 | NC_004680.1 | + | 52926 | 0.68 | 0.405405 |
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Target: 5'- uUGAGUUCCagUCGCauaGCGGCGGCauucuCGUCc -3' miRNA: 3'- -ACUUAGGG--AGCGag-CGCCGUCG-----GCAG- -5' |
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| 18129 | 3' | -59 | NC_004680.1 | + | 42762 | 0.68 | 0.376315 |
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Target: 5'- ----aCCgUCGC-CGCGGCAuccguguaggaggcGCCGUCg -3' miRNA: 3'- acuuaGGgAGCGaGCGCCGU--------------CGGCAG- -5' |
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| 18129 | 3' | -59 | NC_004680.1 | + | 36350 | 1.09 | 0.000456 |
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Target: 5'- uUGAAUCCCUCGCUCGCGGCAGCCGUCu -3' miRNA: 3'- -ACUUAGGGAGCGAGCGCCGUCGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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