Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18129 | 5' | -52.6 | NC_004680.1 | + | 38955 | 0.66 | 0.88167 |
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Target: 5'- gCGGGCgaaCUGCUugGugGAGUcgauGGCg- -3' miRNA: 3'- -GCUUGaa-GACGGugCugCUCA----UCGgg -5' |
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| 18129 | 5' | -52.6 | NC_004680.1 | + | 2830 | 0.66 | 0.873943 |
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Target: 5'- gCGAACUUCUGCCACc-CGuca--CCCg -3' miRNA: 3'- -GCUUGAAGACGGUGcuGCucaucGGG- -5' |
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| 18129 | 5' | -52.6 | NC_004680.1 | + | 53458 | 0.66 | 0.865963 |
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Target: 5'- gGAACUcgUGUCugGCGGCGGGUagguGGCCUu -3' miRNA: 3'- gCUUGAagACGG--UGCUGCUCA----UCGGG- -5' |
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| 18129 | 5' | -52.6 | NC_004680.1 | + | 11194 | 0.66 | 0.857736 |
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Target: 5'- aCGAACggccgcggcgUUCUGCUugGGCGccggGGCCg -3' miRNA: 3'- -GCUUG----------AAGACGGugCUGCuca-UCGGg -5' |
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| 18129 | 5' | -52.6 | NC_004680.1 | + | 40992 | 0.66 | 0.857736 |
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Target: 5'- uCGGGggUCgaGCCugG-UGAGUAGUCCa -3' miRNA: 3'- -GCUUgaAGa-CGGugCuGCUCAUCGGG- -5' |
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| 18129 | 5' | -52.6 | NC_004680.1 | + | 21842 | 0.67 | 0.82253 |
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Target: 5'- ----aUUCgGCCGCGACGAgGUGGCaCUc -3' miRNA: 3'- gcuugAAGaCGGUGCUGCU-CAUCG-GG- -5' |
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| 18129 | 5' | -52.6 | NC_004680.1 | + | 22035 | 0.67 | 0.813198 |
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Target: 5'- aCGcGACUguggGCCACGGCGGccauGCCCa -3' miRNA: 3'- -GC-UUGAaga-CGGUGCUGCUcau-CGGG- -5' |
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| 18129 | 5' | -52.6 | NC_004680.1 | + | 33645 | 0.67 | 0.813198 |
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Target: 5'- aGGAgUUCc-CCACGGCGGGc-GCCCg -3' miRNA: 3'- gCUUgAAGacGGUGCUGCUCauCGGG- -5' |
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| 18129 | 5' | -52.6 | NC_004680.1 | + | 36302 | 1.13 | 0.001189 |
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Target: 5'- uCGAACUUCUGCCACGACGAGUAGCCCa -3' miRNA: 3'- -GCUUGAAGACGGUGCUGCUCAUCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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