Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18131 | 3' | -56.6 | NC_004680.1 | + | 11186 | 0.66 | 0.680575 |
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Target: 5'- gGCGCGUCGGgGucaUCGUcUGGCCCGa- -3' miRNA: 3'- -CGCGUAGUCgUc--AGCGcACUGGGCac -5' |
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| 18131 | 3' | -56.6 | NC_004680.1 | + | 37234 | 0.66 | 0.659027 |
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Target: 5'- aGCGUucCAGcCGGaUUGUGUGGCCCGa- -3' miRNA: 3'- -CGCGuaGUC-GUC-AGCGCACUGGGCac -5' |
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| 18131 | 3' | -56.6 | NC_004680.1 | + | 23440 | 0.66 | 0.637382 |
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Target: 5'- aCGCugAUCGuGCAGgccgccCGCGUGACUgGUGg -3' miRNA: 3'- cGCG--UAGU-CGUCa-----GCGCACUGGgCAC- -5' |
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| 18131 | 3' | -56.6 | NC_004680.1 | + | 54548 | 0.66 | 0.637382 |
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Target: 5'- cGgGCGUCAGCgAGU-GCGUGguGCUCGUc -3' miRNA: 3'- -CgCGUAGUCG-UCAgCGCAC--UGGGCAc -5' |
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| 18131 | 3' | -56.6 | NC_004680.1 | + | 3696 | 0.68 | 0.524022 |
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Target: 5'- uGCGCcucaguUCAGCGGUCGCagccgcauacucagcGUGAUCCa-- -3' miRNA: 3'- -CGCGu-----AGUCGUCAGCG---------------CACUGGGcac -5' |
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| 18131 | 3' | -56.6 | NC_004680.1 | + | 7779 | 0.7 | 0.449541 |
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Target: 5'- cGCGCAggaCGGCAagcuUCGCGaUGACCUGUu -3' miRNA: 3'- -CGCGUa--GUCGUc---AGCGC-ACUGGGCAc -5' |
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| 18131 | 3' | -56.6 | NC_004680.1 | + | 27539 | 0.71 | 0.393652 |
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Target: 5'- cGCGCca-GGCAG-CGCGgGACCCGg- -3' miRNA: 3'- -CGCGuagUCGUCaGCGCaCUGGGCac -5' |
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| 18131 | 3' | -56.6 | NC_004680.1 | + | 36856 | 1.1 | 0.000745 |
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Target: 5'- aGCGCAUCAGCAGUCGCGUGACCCGUGu -3' miRNA: 3'- -CGCGUAGUCGUCAGCGCACUGGGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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