Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1814 | 5' | -56 | NC_001347.2 | + | 56292 | 0.71 | 0.792329 |
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Target: 5'- cGCGCgCACGG-CGCGGUcCCgUGaUGGAg -3' miRNA: 3'- -CGCGaGUGUCuGCGCCAuGG-AC-ACCU- -5' |
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| 1814 | 5' | -56 | NC_001347.2 | + | 150549 | 0.72 | 0.72784 |
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Target: 5'- cGUGCUCuuccaGCAGACGCGGcaUACCUGccugcagcUGGu -3' miRNA: 3'- -CGCGAG-----UGUCUGCGCC--AUGGAC--------ACCu -5' |
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| 1814 | 5' | -56 | NC_001347.2 | + | 47881 | 0.72 | 0.718249 |
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Target: 5'- cGUGgaCGCGGGCGUGGUGguuCgUGUGGAu -3' miRNA: 3'- -CGCgaGUGUCUGCGCCAU---GgACACCU- -5' |
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| 1814 | 5' | -56 | NC_001347.2 | + | 229470 | 0.74 | 0.60007 |
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Target: 5'- cGUGCUCGCuGACGCGGguuugcuuCCUauauaGUGGAc -3' miRNA: 3'- -CGCGAGUGuCUGCGCCau------GGA-----CACCU- -5' |
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| 1814 | 5' | -56 | NC_001347.2 | + | 28636 | 0.76 | 0.484723 |
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Target: 5'- gGCGC-CuCGGGCGCcauGUACCUGUGGAc -3' miRNA: 3'- -CGCGaGuGUCUGCGc--CAUGGACACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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